Analysis Tools
immune system
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• mice are able to clear a Giardia muris infection in a manner similar to wild-type mice
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Allele Symbol Allele Name Allele ID |
Ighmtm1Che targeted mutation 1, Jianzhu Chen MGI:1934014 |
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| Summary |
2 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice are able to clear a Giardia muris infection in a manner similar to wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in spleen of mutants, a 2.6-fold increase in B-1 cells is detected
• in peritoneum, B-1 B cell numbers are elevated ~3-fold at 4 weeks of age through 12 months
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• after LPS challenge, marginal zone metallophilic -2+ (MOMA-2+) macrophages of the T and B cell zone fail to establish a ring of complement 3 (C3)-producing macrophages in the outer B cell zone close to the marginal zone
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• 72 hours after LPS challenge, spleen white pulp area continues to deteriorate; B cell follicles dissolve
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• 7 days after secondary immunization with suboptimal dose of T cell-dependent antigen NP-KLH, number and size of germinal centers in mutant spleens are reduced compared to controls; number is reduced ~4fold, but numbers are normal in mice immunized with 10 or 100 ug of antigen
• germinal center reactions are also impaired when 1 ug of NP-KLH antigen is used for immunization
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• after 72 hours of LPS treatment, marginal zone is no longer structured; rings of 2F8+, ER-TR9+, and MOMA-1+ macrophages are not detectable
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• T cell zone shows severe destruction with disorganized B and T cells 72 hours after LPS treatment
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• germinal center reactions are impaired when 1 ug of NP-KLH antigen is used for immunization
• antibody affinity maturation is compromised in response to suboptimal doses of antigen (NP5-BSA or NP15-BSA)
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• elevated in serum of mutants at young ages
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• IgG1 levels after immunization with a suboptimal dose (<10 ug) of T cell-dependent antigen NP-KLH >100-fold lower in mutants
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• elevated in serum of mutants at 4 weeks, but difference disappears as mice mature
• treatment with the T cell independent antigen NP-Ficoll results in 3-4-fold increase in IgG2a at 7 and 14 days after immunization
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• treatment with the T cell independent antigen NP-Ficoll reduced IgG2b levels after immunization
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• elevated in serum of mutants at 4 weeks, but difference disappears as mice mature
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• IgM levels in serum are ~0.1 ug/ml compared to ~250 ug/ml in serum of wild-type controls; levels do not increase in mice up to 1 year in age
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• antigen trapping on follicular dendritic cells (FDCs) in spleen germinal centers is impaired when 10 ug of NP-KLH antigen is used for immunization; no antigen trapping is detected on FDCs 12 days after immunization, even though titers of NP-specific IgG antibodies are comparable to wild-type
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• treatment with the T cell independent antigen NP-Ficoll results in 3-4-fold increase in IgG2a at 7 and 14 days after immunization
• IgG responses to T cell-dependent antigen NP-KLH are impaired at suboptimal antigen doses; at a suboptimal dose 7 days after immunization, IgG antibody levels are ~25-fold lower for primary and secondary responses relative to heterozygous controls
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• in spleen of mutants, a 2.6-fold increase in B-1 cells is detected
• in peritoneum, B-1 B cell numbers are elevated ~3-fold at 4 weeks of age through 12 months
|
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• after LPS challenge, marginal zone metallophilic -2+ (MOMA-2+) macrophages of the T and B cell zone fail to establish a ring of complement 3 (C3)-producing macrophages in the outer B cell zone close to the marginal zone
|
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• 72 hours after LPS challenge, spleen white pulp area continues to deteriorate; B cell follicles dissolve
|
|
• 7 days after secondary immunization with suboptimal dose of T cell-dependent antigen NP-KLH, number and size of germinal centers in mutant spleens are reduced compared to controls; number is reduced ~4fold, but numbers are normal in mice immunized with 10 or 100 ug of antigen
• germinal center reactions are also impaired when 1 ug of NP-KLH antigen is used for immunization
|
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• after 72 hours of LPS treatment, marginal zone is no longer structured; rings of 2F8+, ER-TR9+, and MOMA-1+ macrophages are not detectable
|
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• T cell zone shows severe destruction with disorganized B and T cells 72 hours after LPS treatment
|
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• elevated in serum of mutants at young ages
|
|
• IgG1 levels after immunization with a suboptimal dose (<10 ug) of T cell-dependent antigen NP-KLH >100-fold lower in mutants
|
|
• elevated in serum of mutants at 4 weeks, but difference disappears as mice mature
• treatment with the T cell independent antigen NP-Ficoll results in 3-4-fold increase in IgG2a at 7 and 14 days after immunization
|
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• treatment with the T cell independent antigen NP-Ficoll reduced IgG2b levels after immunization
|
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• elevated in serum of mutants at 4 weeks, but difference disappears as mice mature
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• IgM levels in serum are ~0.1 ug/ml compared to ~250 ug/ml in serum of wild-type controls; levels do not increase in mice up to 1 year in age
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/30/2024 MGI 6.23 |
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