Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cr2tm1Crr mutation
(0 available);
any
Cr2 mutation
(84 available)
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hematopoietic system
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• absolute numbers of follicular B cells are decreased
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• absolute numbers of marginal zone B cells are increased
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immune system
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• absolute numbers of follicular B cells are decreased
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• absolute numbers of marginal zone B cells are increased
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Allelic
Composition |
Cr2tm1Crr/Cr2tm1Crr
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Genetic
Background |
involves: 129S7/SvEvBrd * C57BL/6 |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cr2tm1Crr mutation
(0 available);
any
Cr2 mutation
(84 available)
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mortality/aging
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• following cecal ligation and puncture
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immune system
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N |
• B cells show normal responses to LPS, IgM and CD40L
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• productive:nonproductive ratios of Ig heavy chain rearrangement are lower than in wild-type; somatic hypermutation is not reduced in mutants however
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• in the peritoneal lavage following cecal ligation and puncture
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• Cr2-deficient mice show a reduction in peritoneal B-1a (IgM+, CD5+) cells compared to wild-type (42% vs 71%)
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• 7 days after secondary challenge with phage, wild-type mice show >50% of splenic follicles containing germinal centers compared to many fewer in Cr2-deficient mice
(J:31942)
• in Cr2-deficient mice, immunization with 5 ug of antigen results in 5-fold decrease in germinal center formation compared to wild-type receiving the same dose
(J:69452)
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• germinal centers exhibit cross-sectional areas about 10-fold less than in wild-type controls
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• mutants show defective humoral immune response to T dependent antigens
(J:31942)
• after immunization with bacteriophage phiX174, Cr2-deficient mice fail to make normal primary or secondary humoral immune responses; IgG titers are reduced in mutants one week after immunization compared to controls
(J:31942)
• bone marrow chimeras (wild-type bone marrow in Cr2-deficient hosts) have normal primary and secondary humoral responses after phage immunization
(J:31942)
• Cr2-deficient mice have a weaker response to intrapertioneal injection with NP-CG compared to wild-type
(J:69452)
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• antibody responses decay twice as fast in Cr2-deficient mice as controls
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• igG2a, 1gG2b and IgG3 levels are reduced in mutants compared to wild-type
(J:31942)
• 5 ug NP-CG elicits a 4-fold weaker IgG1 response in Cr2-deficient mice; 50 ug NP-CG raises levels to half that of controls and a 10-fold increase in antigen dose results in a 7-fold increase in IgG1 response
(J:69452)
• serum IgG1 levels are comparable in mutants and controls until day 16 post-immunization, then loss in mutants is accelerated
(J:69452)
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• 5 ug NP-CG elicits a 3-fold weaker IgM response in Cr2-deficient mice; a 10-fold increase in antigen results in a 3-fold increase in IgM
• after day 6 following immunization, serum IgM levels drop more rapidly in mutants compared to controls
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• in the peritoneal lavage following cecal ligation and puncture
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homeostasis/metabolism
hematopoietic system
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• in the peritoneal lavage following cecal ligation and puncture
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• Cr2-deficient mice show a reduction in peritoneal B-1a (IgM+, CD5+) cells compared to wild-type (42% vs 71%)
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• 7 days after secondary challenge with phage, wild-type mice show >50% of splenic follicles containing germinal centers compared to many fewer in Cr2-deficient mice
(J:31942)
• in Cr2-deficient mice, immunization with 5 ug of antigen results in 5-fold decrease in germinal center formation compared to wild-type receiving the same dose
(J:69452)
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• germinal centers exhibit cross-sectional areas about 10-fold less than in wild-type controls
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• antibody responses decay twice as fast in Cr2-deficient mice as controls
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• igG2a, 1gG2b and IgG3 levels are reduced in mutants compared to wild-type
(J:31942)
• 5 ug NP-CG elicits a 4-fold weaker IgG1 response in Cr2-deficient mice; 50 ug NP-CG raises levels to half that of controls and a 10-fold increase in antigen dose results in a 7-fold increase in IgG1 response
(J:69452)
• serum IgG1 levels are comparable in mutants and controls until day 16 post-immunization, then loss in mutants is accelerated
(J:69452)
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• 5 ug NP-CG elicits a 3-fold weaker IgM response in Cr2-deficient mice; a 10-fold increase in antigen results in a 3-fold increase in IgM
• after day 6 following immunization, serum IgM levels drop more rapidly in mutants compared to controls
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Allelic
Composition |
Cr2tm1Crr/Cr2tm1Crr
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Genetic
Background |
involves: 129S7/SvEvBrd * C57BL/6J |
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cr2tm1Crr mutation
(0 available);
any
Cr2 mutation
(84 available)
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immune system
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• at 5-6 months of age, frequency of anti-dsDNA antibodies is significantly increased in female Cr2-deficient mice compared to B6.129-Ighb controls
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cr2tm1Crr mutation
(0 available);
any
Cr2 mutation
(84 available)
Ggta1tm1Jbl mutation
(1 available);
any
Ggta1 mutation
(80 available)
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immune system
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• double mutants do not produce alpha-Gal-reactive natural antibodies; Ggta1-null and double mutants given anti alpha-Gal serumproduce similar levels of BSA-specific IgG antibodies after alpha-Gal immunization, indicating that complement receptors are not required to produce increased carrier immunogenicity
• elevated BSA immunogenicity in mutants is directly related to alpha-Gal specific antibodies
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Analysis Tools