Phenotypes associated with this allele
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En2tm2Alj mutation
(0 available);
any
En2 mutation
(104 available)
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nervous system
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• abnormal folding pattern
• fused folds in the hemispheres results in 3 rather than 4 lobules
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• altered pattern
• 3 rather than 4 major folds
• paraflocculus about half normal size
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• lobe VIII is associated with lobe IX rather than lobes VI and VII
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Allelic Composition |
En2tm2Alj/En2tm2Alj
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Genetic Background |
involves: 129S2/SvPas * Swiss Webster |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En2tm2Alj mutation
(0 available);
any
En2 mutation
(104 available)
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nervous system
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• reduced in size at E18.5
• in adults, IC is truncated
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• size is slightly reduced at E11.5
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• in E18.5 mutants, there is a general delay in fissure formation in vermis
• in adults, vermis is reduced in size
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• folium VIII position is shifted posteriorly and is associated with folium IX instead of with VI/VII
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• overall size reduced by one-third
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Allelic Composition |
En2tm2Alj/En2tm3Alj
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Genetic Background |
involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6 |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En2tm2Alj mutation
(0 available);
any
En2 mutation
(104 available)
En2tm3Alj mutation
(0 available);
any
En2 mutation
(104 available)
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nervous system
N |
• brain morphology is normal
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nervous system
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• reduced in size at E18.5
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• at E18.5, no cerebellum is present
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nervous system
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• at E18.5, no cerebellum is present
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nervous system
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• when tamoxifen is administered at E10.5, rhombomere 1 (r1) is reduced in size in mutants at E12.5
• anterior r1 cells contribute to more lateral regions of vermis than normal
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• when tamoxifen is administered at E10.5, mesencephalon is reduced in size at E12.5 relative to normal
• marked cells in posterior mesencephalon do not expand normally
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• when tamoxifen is administered at E10.5, size of marked domain is smaller than wild-type at E16.5
• in adults, marked domain in tectum is greatly reduced compared to wild-type; marked domain is restricted to remaining region of inferior colliculus
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• when tamoxifen is administered at E10.5, size of marked cell population is wider in mutants than in controls at E16.5
• in adults, marked domain is broader than normal
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• in adults, vermis is reduced in size
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embryo
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• anterior r1 cells contribute to more lateral regions of vermis than normal
• when tamoxifen is administered at E10.5, rhombomere 1 (r1) is reduced in size in mutants at E12.5
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1tm2Alj mutation
(1 available);
any
En1 mutation
(32 available)
En2tm2Alj mutation
(0 available);
any
En2 mutation
(104 available)
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nervous system
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• most or all of r1 deleted by E9.5
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• most or all of r1 deleted by E9.5
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• development normal through early somite stage but abnormal by 10-12 somite stage
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• general deletion of the mesencephalon/anterior metencephalon region at E9.5
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• mostly, if not entirely, deleted at E9.5
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• reduced in overall size by one-third at E18.5
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embryo
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• most or all of r1 deleted by E9.5
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• most or all of r1 deleted by E9.5
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1tm2Alj mutation
(1 available);
any
En1 mutation
(32 available)
En2tm2Alj mutation
(0 available);
any
En2 mutation
(104 available)
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mortality/aging
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• no mutants are detected after birth
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nervous system
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• at E18.5, no cerebellum is observed
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Allelic Composition |
En1tm2Alj/En1+ En2tm2Alj/En2+
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Genetic Background |
involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1tm2Alj mutation
(1 available);
any
En1 mutation
(32 available)
En2tm2Alj mutation
(0 available);
any
En2 mutation
(104 available)
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nervous system
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• delay in cerebellum foliation is observed in adult mice
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• in E18.5 mutants, there is a general delay in fissure formation in vermis; less severe than in En2tm2Alj homozygotes
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• vermis displays fused folia, and folia I, II, and III are smaller than normal in adults
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• folium VIII is positioned between lobule VII and IX; folium VIII position is shifted posteriorly but less than in En2tm2Alj homozygotes
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1tm2Alj mutation
(1 available);
any
En1 mutation
(32 available)
En2tm2Alj mutation
(0 available);
any
En2 mutation
(104 available)
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mortality/aging
N |
• mutants survive to adulthood
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nervous system
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• severely truncated in one mutant and fused with folia I-V remnants
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• at E18.5, absent in 1 mutant
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• at E18.5, structure is absent (1 mutant) or only small amount of tissue is present
• partially truncated in adults
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• at E11.5, truncation of tectum is greater than in En2tm2Alj homozygotes
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• in posterior hemisphere crusil and paramedian folia are fused; in one embryo, a partial fissure separating crusil and paramedian folia is observed at E18.5
• hemispheres are reduced in size compared to En2tm2Alj adult homozygotes
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• delay in cerebellum foliation is more severe than in En2tm2Alj homozygotes
• there are more foliation defects adults than in En2tm2Alj adult homozygotes or or En1tm2Alj / En2tm2Alj adult double heterozygotes
• in most mutants, the five anterior-most folia (I-V) are replaced by a single fold
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• only 1 distinct folium is present in anterior vermis
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• substantial deletion of folium VIII is detected in adults
• in some mutants, folium VIII is absent or reduced in size and misaligned with folium IX; in one mutant folia I-V are almost completely absent and fused to profoundly truncated tectum
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• at E18.5, no cerebellum is observed in some mutants
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• reduced in overall size by half at E18.5 in some mutants
• size reduction in adults is greater than in En2tm2Alj or En1tm2Alj / En2tm2Alj adult heterozygotes
• size reduction at E11.5 is greater than in En2tm2Alj homozygotes
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1tm5(en)Alj mutation
(0 available);
any
En1 mutation
(32 available)
En2tm2Alj mutation
(0 available);
any
En2 mutation
(104 available)
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mortality/aging
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• no mutants are detected after birth
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nervous system
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• structure does not form
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• in some mutants, structure does not form
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• in some mutants structure is half normal size
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• at E18.5, no cerebellum is observed
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1tm5(en)Alj mutation
(0 available);
any
En1 mutation
(32 available)
En2tm2Alj mutation
(0 available);
any
En2 mutation
(104 available)
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mortality/aging
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• about half of the expected number of mutants survive to adulthood
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nervous system
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• reduced in size in surviving adults
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• vermis displays fused folia, and folia I, II, and III are smaller than normal in adults
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• subregion VIII (posterior vermis) is not present in adults
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• in adults, reduced in overall size by one-third relative to controls
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1tm5(en)Alj mutation
(0 available);
any
En1 mutation
(32 available)
En2tm2Alj mutation
(0 available);
any
En2 mutation
(104 available)
|
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nervous system
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• reduced in size at E18.5
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• in posterior hemisphere crusil and paramedian folia are fused; in one embryo, a partial fissure separating crusil and paramedian folia is observed
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• foliation defects are milder than in En1tm2Alj /+, En2tm2Alj homozygotes; most mutants have foliation pattern similar to hemispheres of En2tm2Alj homozygotes
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• subregion VIII (posterior vermis) is not present at E18.5
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• at E18.5, reduced in overall size by one-third relative to controls
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1tm2Alj mutation
(1 available);
any
En1 mutation
(32 available)
En1tm3(En2)Alj mutation
(0 available);
any
En1 mutation
(32 available)
En2tm2Alj mutation
(0 available);
any
En2 mutation
(104 available)
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nervous system
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• reduced in size at E18.5
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• in posterior hemisphere crusil and paramedian folia are fused; in one embryo, a partial fissure separating crusil and paramedian folia is observed
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• foliation defects are milder than in En1tm2Alj /+, En2tm2Alj homozygotes; most mutants have foliation pattern similar to hemispheres of En2tm2Alj homozygotes
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• subregion VIII (posterior vermis) is not present at E18.5
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• at E18.5, reduced in overall size by one-third relative to controls
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1tm3(En2)Alj mutation
(0 available);
any
En1 mutation
(32 available)
En2tm2Alj mutation
(0 available);
any
En2 mutation
(104 available)
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mortality/aging
N |
• mice are viable into adulthood
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nervous system
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• in posterior hemisphere crusil and paramedian folia are fused
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• in a small number of mutants, folium VIII is abnormal, being associated with folium IX
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• at E18.5, reduced in overall size by one-third relative to controls
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
En1tm3(En2)Alj mutation
(0 available);
any
En1 mutation
(32 available)
En2tm2Alj mutation
(0 available);
any
En2 mutation
(104 available)
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nervous system
N |
• cerebellum and colliculi have normal morphology at E18.5
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