Phenotypes associated with this allele
|
Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Trp53tm1Wahl mutation
(0 available);
any
Trp53 mutation
(232 available)
|
|
|
cellular
|
• MEFs do not arrest like wild-type and continue to cycle in response to gamma-irradiation or nucleotide pool depletion via PALA, an inhibitor of aspartate transcarbamylase (low G1/S ratio)
(J:64367)
• embryonic fibroblasts from Trp53-deficient mice display a lack of cell cycle arrest upon exposure to increasing doses of ionizing radiation
(J:100189)
|
|
• MEFs do not arrest like wild-type and continue to cycle in response to gamma-irradiation
• less than 1% of thymocytes from irradiated mutants undergo apoptosis compared to 35-60% of wild-type thymocytes
|
|
• MEFs continue to proliferate past the point when normal MEFs undergo replicative senescence
|
|
Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Trp53tm1Wahl mutation
(0 available);
any
Trp53 mutation
(232 available)
|
|
|
cellular
|
• MEFs sensitized to apoptosis by E1A overexpression show very low levels of apoptosis induced by irradiation or chemical treatment compared to wild-type or Trp53tm1Wahl MEFs
|
neoplasm
|
• knockouts spontaneously develop fatal tumors by 10 months of age
|
|
Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mdm2tm1Glo mutation
(0 available);
any
Mdm2 mutation
(54 available)
Mdm4Gt(VICTR20)7Lex mutation
(0 available);
any
Mdm4 mutation
(191 available)
Trp53tm1Wahl mutation
(0 available);
any
Trp53 mutation
(232 available)
|
|
|
cellular
|
• presence of one copy of Mdm2 partially restores the arrest response of Trp53tm1Wahl; p21 protein levels are increased slightly more compared to homozygous Trp53tm1Wahl, Mdm2tm1Glo/+ or homozygous Trp53tm1Wahl, Mdm4/+ heterozygotes
|
|
Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mdm4Gt(VICTR20)7Lex mutation
(0 available);
any
Mdm4 mutation
(191 available)
Trp53tm1Wahl mutation
(0 available);
any
Trp53 mutation
(232 available)
|
|
|
cellular
|
• in double homozygous MEFs, irradiation did result in cell cycle arrest, but less efficiently than in wild-type cells; p21 protein levels are the highest of all the Trp53tm1Wahl mutants analyzed
|
|
• loss of Mdm4 expression reduces the proliferation rate of Trp53tm1Wahl-deficient cells
|
|
Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mdm4Gt(VICTR20)7Lex mutation
(0 available);
any
Mdm4 mutation
(191 available)
Trp53tm1Wahl mutation
(0 available);
any
Trp53 mutation
(232 available)
|
|
|
cellular
|
• presence of one copy of Mdm2 partially restores the arrest response of Trp53tm1Wahl; p21 protein levels are modestly increased from homozygous Trp53tm1Wahl mutant levels
|
|
Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mdm2tm1Glo mutation
(0 available);
any
Mdm2 mutation
(54 available)
Trp53tm1Wahl mutation
(0 available);
any
Trp53 mutation
(232 available)
|
|
|
mortality/aging
|
• no double homozygotes survive to birth
|
|
Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mdm2tm1Glo mutation
(0 available);
any
Mdm2 mutation
(54 available)
Trp53tm1Wahl mutation
(0 available);
any
Trp53 mutation
(232 available)
|
|
|
cellular
|
• presence of one copy of Mdm2 partially restores the arrest response of Trp53tm1Wahl; p21 protein levels are modestly increased from homozygous Trp53 mutant levels
|