All Phenotypes Chrm3<tm1Mmt> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Chrm3^tm1Mmt
targeted mutation 1, Makoto M Taketo
MGI:1888740

Summary

5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Chrm3^tm1Mmt/Chrm3^tm1Mmt	B6.129X1-Chrm3^tm1Mmt	MGI:4443054
hm2	Chrm3^tm1Mmt/Chrm3^tm1Mmt	involves: 129X1/SvJ	MGI:3581818
hm3	Chrm3^tm1Mmt/Chrm3^tm1Mmt	involves: 129X1/SvJ * C57BL/6	MGI:3581978
cx4	Chrm2^tm1Minm/Chrm2^tm1Minm Chrm3^tm1Mmt/Chrm3^tm1Mmt	involves: 129X1/SvJ * C57BL/6	MGI:3581821
cx5	Chrm1^tm1Kano/Chrm1^tm1Kano Chrm3^tm1Mmt/Chrm3^tm1Mmt	involves: 129X1/SvJ * C57BL/6	MGI:3581877

Allelic Composition	Chrm3^tm1Mmt/Chrm3^tm1Mmt
Genetic Background	B6.129X1-Chrm3^tm1Mmt

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chrm3^tm1Mmt mutation (0 available); any Chrm3 mutation (50 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

decreased digestive secretion ( J:104782 )

• although there is a small increase in gastric acid secretion in response to carbachol, it is significantly decreased compared to wild type

• gastric mucosal histology is normal

homeostasis/metabolism

abnormal histamine physiology ( J:104782 )

• carbachol-stimulated histamine secretion is decreased compared with stimulated secretion in wild type

Allelic Composition	Chrm3^tm1Mmt/Chrm3^tm1Mmt
Genetic Background	involves: 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chrm3^tm1Mmt mutation (0 available); any Chrm3 mutation (50 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

muscle

impaired smooth muscle contractility ( J:99247 )

• bladder and ileal smooth muscle do not respond to carbachol, even at concentrations that evoke maximal cholinergic contractions in wild-type

impaired contractility of ileal smooth muscle ( J:99247 )

• ileal smooth muscle does not respond to carbachol, even at concentrations that evoke maximal cholinergic contractions in wild-type

vision/eye

mydriasis ( J:99247 )

• partially dilated pupils

nervous system

abnormal inhibitory postsynaptic currents ( J:98902 )

• hippocampal neuron pairs exhibit a reduction in the enhanced depolarization-induced suppression of inhibitory (DSI) postsynaptic currents in response to the muscarinic agonist oxotremorine-M, however it persisted to a substantial degree

behavior/neurological

increased fluid intake ( J:105126 )

• exhibit an increase in frequency of prandial drinking due to dryness of food as this effect is not seen with hydrated paste food

digestive/alimentary system

abnormal submandibular gland physiology ( J:105126 )

• carbachol-induced intracellular calcium concentration increase is markedly impaired in submandibular gland cells, however phenylephrine, an alpha1-adrenergic agonist, induces similar calcium responses as in wild-type

decreased salivation ( J:105126 )

• salivary secretion is reduced in response to low levels of pilocarpine and although higher levels of pilocarpine induce considerable salivation, it is still less than in wild-type

• rate of saliva secretion is significantly lower than in wild-type during the early period after pilocarpine administration

endocrine/exocrine glands

abnormal submandibular gland physiology ( J:105126 )

decreased salivation ( J:105126 )

• salivary secretion is reduced in response to low levels of pilocarpine and although higher levels of pilocarpine induce considerable salivation, it is still less than in wild-type

• rate of saliva secretion is significantly lower than in wild-type during the early period after pilocarpine administration

homeostasis/metabolism

decreased salivation ( J:105126 )

• salivary secretion is reduced in response to low levels of pilocarpine and although higher levels of pilocarpine induce considerable salivation, it is still less than in wild-type

• rate of saliva secretion is significantly lower than in wild-type during the early period after pilocarpine administration

Allelic Composition	Chrm3^tm1Mmt/Chrm3^tm1Mmt
Genetic Background	involves: 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chrm3^tm1Mmt mutation (0 available); any Chrm3 mutation (50 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Pupil appearance of Chrm3^tm1Mmt/⁺ and Chrm3^tm1Mmt/Chrm3^tm1Mmt mice.

growth/size/body

decreased body size ( J:64069 )

• smaller at around 3 weeks of age

decreased body weight ( J:64069 )

• average body weight is only half that of controls at 4-5 weeks of age, however by 15 weeks of age, homozygous males catch up with controls while homozygous females remain smaller and are about 80% of wild-type weight

vision/eye

mydriasis ( J:64069 )

• exhibit dilated ocular pupils, however find no other eye abnormalities

• no change in pupil size in response to pilocarpine, a nonselective muscarinic agonist, however atropine instillation causes further dilation and completed mydriasis

renal/urinary system

impaired contractility of urinary bladder detrusor smooth muscle ( J:64069 )

• contractility of the detrusor muscles by carbachol, but not KCl, stimulation is decreased to only 5% of that seen in wild-type

hydronephrosis ( J:64069 )

• mild hydronephrosis in 3 of 10 homozygous males, showing a distended renal calyx with some perivascular lymphocyte infiltrations in the adjacent medulla

abnormal urinary bladder morphology ( J:64069 )

• bladder wall is thinner because of the severe distension and the submucosal layer contains some focal lymphocyte infiltrations in homozygous males, while the urothelium and renal function appear normal

distended urinary bladder ( J:64069 )

• severely distended in males and mildly distended in females

muscle

impaired contractility of ileal smooth muscle ( J:64069 )

• maximal contraction of ileal smooth muscle in response to carbachol, but not KCl, is decreased by 77% in males and by 72% in females

impaired contractility of urinary bladder detrusor smooth muscle ( J:64069 )

• contractility of the detrusor muscles by carbachol, but not KCl, stimulation is decreased to only 5% of that seen in wild-type

digestive/alimentary system

decreased salivation ( J:64069 )

• salivary secretion is abolished in response to pilocarpine but not to isoproterenol, a beta-adrenergic agonist

endocrine/exocrine glands

decreased salivation ( J:64069 )

• salivary secretion is abolished in response to pilocarpine but not to isoproterenol, a beta-adrenergic agonist

homeostasis/metabolism

decreased salivation ( J:64069 )

• salivary secretion is abolished in response to pilocarpine but not to isoproterenol, a beta-adrenergic agonist

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
megacystis-microcolon-intestinal hypoperistalsis syndrome		DOID:0060610	OMIM:155310	J:64069

Allelic Composition	Chrm2^tm1Minm/Chrm2^tm1Minm Chrm3^tm1Mmt/Chrm3^tm1Mmt
Genetic Background	involves: 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chrm2^tm1Minm mutation (0 available); any Chrm2 mutation (32 available)
Chrm3^tm1Mmt mutation (0 available); any Chrm3 mutation (50 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

postnatal growth retardation ( J:99247 )

• transient postweaning growth retardation, which is improved by feeding with paste food

muscle

impaired smooth muscle contractility ( J:99247 )

• bladder and ileal smooth muscle do not respond to carbachol, even at concentrations that evoke maximal cholinergic contractions in wild-type

• noncholinergic contraction elicited by electrical field stimulation is significantly weaker when male bladder strips are stimulated at 1 or 10 Hz and is significantly larger when male ileum is stimulated at 1 Hz but not 10 Hz compared to wild-type

• noncholinergic contraction elicited by electrical field stimulation is significantly larger when female bladder strips are stimulated at 1 Hz but not 10 Hz and when female ileum is stimulated at 10 Hz but not 1 Hz compared to wild-type

impaired contractility of ileal smooth muscle ( J:99247 )

• ileal smooth muscle does not respond to carbachol, even at concentrations that evoke maximal cholinergic contractions in wild-type

renal/urinary system

distended urinary bladder ( J:99247 )

• seen only in males and no different from single homozygous Chrm3 mutant mice, however digestive tract, heart, lung and spleen were normal

vision/eye

mydriasis ( J:99247 )

• partially dilated pupils, however they are smaller than in single homozygous Chrm3 mutant mice

Allelic Composition	Chrm1^tm1Kano/Chrm1^tm1Kano Chrm3^tm1Mmt/Chrm3^tm1Mmt
Genetic Background	involves: 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chrm1^tm1Kano mutation (0 available); any Chrm1 mutation (26 available)
Chrm3^tm1Mmt mutation (0 available); any Chrm3 mutation (50 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal inhibitory postsynaptic currents ( J:98902 )

• hippocampal neuron pairs do not exhibit an enhanced depolarization-induced suppression of inhibitory (DSI) postsynaptic currents in response to the muscarinic agonist oxotremorine-M as was seen in controls

digestive/alimentary system

abnormal submandibular gland physiology ( J:105126 )

• carbachol-induced intracellular calcium concentration increase is completely absent in submandibular gland cells, however phenylephrine, an alpha1-adrenergic agonist, induces similar calcium responses as in wild-type

decreased salivation ( J:105126 )

• a high dose of pilocarpine induces almost no salivation compared to wild-type

endocrine/exocrine glands

abnormal submandibular gland physiology ( J:105126 )

decreased salivation ( J:105126 )

• a high dose of pilocarpine induces almost no salivation compared to wild-type

homeostasis/metabolism

decreased salivation ( J:105126 )

• a high dose of pilocarpine induces almost no salivation compared to wild-type

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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