Phenotypes associated with this allele

• Allele Symbol: Pou3f2^tm1Rsd
• Allele Name: targeted mutation 1, Michael G Rosenfeld
• Allele ID: MGI:1857996
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pou3f2^tm1Rsd/Pou3f2^tm1Rsd	involves: 129S4/SvJae * C57BL/6	MGI:2660736
cx2	Pou3f2^tm1Rsd/Pou3f2^tm1Rsd Pou3f3^tm1Rmc/Pou3f3^tm1Rmc	involves: 129S4/SvJae * C57BL/6	MGI:2660729

Genotype
MGI:2660736

hm1

• Allelic Composition: Pou3f2^tm1Rsd/Pou3f2^tm1Rsd
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:30430 )

• 90% of homozygotes died before P6

• the remaining 10% died between P9 and P10

adipose tissue

decreased brown adipose tissue amount ( J:30430 )

• most homozygotes showed a significant decrease in brown adipose tissue, consistent with severe malnutrition

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:30430 )

N • the adrenal glands and cerebral cortex of homozygous mutant mice appeared normal

abnormal pituitary gland morphology ( J:30430 )

• by E19, homozygotes displayed complete loss of pituicytes and a fold of the intermediate lobe filled the space normally occupied by the posterior lobe

abnormal neurohypophysis morphology ( J:30430 )

• homozygotes displayed loss of the posterior lobe of the pituitary gland; the anterior and intermediate lobes appeared normal

• the developing posterior pituitary gland appeared normal until E16.5, when axonal projections from magnocellular neurons failed to make contact

abnormal pituicyte morphology ( J:30430 )

• complete loss of pituicytes by E19

growth/size/body

decreased body size ( J:30430 )

• homozygotes were born normally but displayed a reduction in size and weight from P3

• by P6, mutants were 50-60% smaller than age-matched wild-type mice

nervous system

nervous system phenotype ( J:30430 )

N • homozygotes showed no abnormalities in the cortex, hippocampal formation, or other structures outside the hypothalamus

abnormal pituitary gland morphology ( J:30430 )

• by E19, homozygotes displayed complete loss of pituicytes and a fold of the intermediate lobe filled the space normally occupied by the posterior lobe

abnormal neurohypophysis morphology ( J:30430 )

• homozygotes displayed loss of the posterior lobe of the pituitary gland; the anterior and intermediate lobes appeared normal

• the developing posterior pituitary gland appeared normal until E16.5, when axonal projections from magnocellular neurons failed to make contact

abnormal pituicyte morphology ( J:30430 )

• complete loss of pituicytes by E19

abnormal hypothalamus morphology ( J:30430 )

• homozygotes displayed loss of endocrine hypothalamic nuclei

• starting at E15-E16, mutants showed a progressive loss of the parvocellular and magnocellular neurons of the paraventricular hypothalamic (PVH) and supraoptic nuclei in a dorsal-ventral pattern, with most cells lost by E17

• by E19.5, mutant neonates showed a striking neuronal loss only in the hypothalamic supraoptic and paraventricular nuclei

integument

hyperkeratosis ( J:30430 )

• by P6, mutants displayed epidermal hyperkeratosis

flaky skin ( J:30430 )

• by P6, mutants had a flaking epidermis

Genotype
MGI:2660729

cx2

• Allelic Composition: Pou3f2^tm1Rsd/Pou3f2^tm1Rsd; Pou3f3^tm1Rmc/Pou3f3^tm1Rmc
• Genetic Background: involves: 129S4/SvJae * C57BL/6

mortality/aging

perinatal lethality, complete penetrance ( J:74989 )

• double homozygotes were not viable at birth

nervous system

abnormal cerebellum development ( J:74989 )

abnormal forebrain morphology ( J:74989 )

• double homozygotes displayed abnormalities in axonal outgrowth and projection in the forebrain

abnormal olfactory bulb development ( J:74989 )

abnormal cerebral cortex morphology ( J:74989 )

• double homozygotes displayed abnormal organization of cortical lamination with delayed radial migration of layers II/III

abnormal stratification in cerebral cortex ( J:74989 )

• double homozygotes displayed inversion of cortical layers II-V through aberrant regulation of the p39/p35 regulatory components of CDK5