Phenotypes associated with this allele

• Allele Symbol: Notch2^tm1Yha
• Allele Name: targeted mutation 1, Yoshio Hamaha
• Allele ID: MGI:1857783
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Notch2^tm1Yha/Notch2^tm1Yha	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:2175160
ht2	Notch2^tm1Yha/Notch2^+	B6.129-Notch2^tm1Yha	MGI:3713687

Genotype
MGI:2175160

hm1

• Allelic Composition: Notch2^tm1Yha/Notch2^tm1Yha
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:55841 , J:120082 )

• die at or around E11.5 (J:55841)

embryo

increased embryonic tissue cell apoptosis ( J:55841 )

• embryos show widespread apoptotic cell death at E9.5, prior to developmental retardation

embryonic growth retardation ( J:55841 , J:120082 )

• developmentally retarded at E10.5 (J:55841)

abnormal placenta morphology ( J:120082 )

abnormal placenta vasculature ( J:120082 )

• inadequate maternal blood sinus formation at E9 and degeneration of fetal blood vessels in E10.5 placentas

• most maternal blood cells appear to remain at the level of the giant cell layer in the placenta and show impaired entry into both spongiotrophoblast and labyrinth layers

abnormal chorionic plate morphology ( J:120082 )

• poor expansion of the chorionic plate in placenta at E9.5

abnormal placenta labyrinth morphology ( J:120082 )

• placenta is thicker, especially in the labyrinth layer

abnormal placenta size ( J:120082 )

• placenta is thicker, likely due to a poor lateral expansion of the chorionic plate

growth/size/body

embryonic growth retardation ( J:55841 , J:120082 )

• developmentally retarded at E10.5 (J:55841)

cellular

increased embryonic tissue cell apoptosis ( J:55841 )

• embryos show widespread apoptotic cell death at E9.5, prior to developmental retardation

cardiovascular system

abnormal placenta vasculature ( J:120082 )

• inadequate maternal blood sinus formation at E9 and degeneration of fetal blood vessels in E10.5 placentas

• most maternal blood cells appear to remain at the level of the giant cell layer in the placenta and show impaired entry into both spongiotrophoblast and labyrinth layers

Genotype
MGI:3713687

ht2

• Allelic Composition: Notch2^tm1Yha/Notch2⁺
• Genetic Background: B6.129-Notch2^tm1Yha

immune system

decreased B-1 B cell number ( J:85379 )

• reduction in B-1 B cells of the peritoneal cavity

abnormal spleen marginal zone morphology ( J:85379 )

• impaired development of marginal zone B cells results in a severe reduction in marginal zone B cells of the spleen

• however, splenic architecture is grossly normal

decreased marginal zone B cell number ( J:85379 )

• severe reduction in marginal zone B cells of the spleen

hematopoietic system

decreased B-1 B cell number ( J:85379 )

• reduction in B-1 B cells of the peritoneal cavity

abnormal spleen marginal zone morphology ( J:85379 )

• impaired development of marginal zone B cells results in a severe reduction in marginal zone B cells of the spleen

• however, splenic architecture is grossly normal

decreased marginal zone B cell number ( J:85379 )

• severe reduction in marginal zone B cells of the spleen