Phenotypes associated with this allele

• Allele Symbol: Ngf⁺
• Allele Name: wild type
• Allele ID: MGI:1857606
• Summary: 8 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Ngf^tm1.1Blhe/Ngf^+	B6.Cg-Ngf^tm1.1Blhe	MGI:5461510
ht2	Ngf^tm2.1Blhe/Ngf^+	B6.Cg-Ngf^tm2.1Blhe	MGI:5461514
ht3	Ngf^tm1(NGF*)Cat/Ngf^+	involves: 129S1/Sv * 129X1/SvJ	MGI:6388458
ht4	Ngf^tm1Gne/Ngf^+	involves: 129S7/SvEvBrd	MGI:6388459
ht5	Ngf^tm1Gne/Ngf^+	involves: 129S7/SvEvBrd * C57BL/6	MGI:2175145
cx6	Ngf^tm1.1Blhe/Ngf^+ Ngfr^tm1Jae/Ngfr^tm1Jae	B6.Cg-Ngf^tm1.1Blhe Ngfr^tm1Jae	MGI:5461512
cx7	Ngf^tm1.1Blhe/Ngf^+ Sort1^tm1Tew/Sort1^tm1Tew	B6.Cg-Ngf^tm1.1Blhe Sort1^tm1Tew	MGI:5461513
cx8	Ngf^tm1.1Blhe/Ngf^+ Tg(Cspg4-DsRed.T1)1Akik/0	B6.Cg-Ngf^tm1.1Blhe Tg(Cspg4-DsRed.T1)1Akik	MGI:5461511

Genotype
MGI:5461510

ht1

• Allelic Composition: Ngf^tm1.1Blhe/Ngf⁺
• Genetic Background: B6.Cg-Ngf^tm1.1Blhe

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Adult Ngf^tm1.1Blhe/Ngf⁺ mice exhibit dilated cardiomyopathy and fibrosis and this phentoype is rescued in Ngfr^tm1Jae/Ngfr^tm1Jae Ngf^tm1.1Blhe/Ngf⁺ mice

mortality/aging

premature death ( J:190893 )

• some mice die by 5 months

• most mice die before 8 months

cardiovascular system

abnormal vascular endothelial cell morphology ( J:190893 )

• many endothelial cells exhibit attenuated cytoplasm with occasional overt rupture and extravasation of erythrocytes unlike in wild-type mice

abnormal myocardium layer morphology ( J:190893 )

• myocardial fibrosis at 8 month without myocyte cell death

abnormal myocardial fiber morphology ( J:190893 )

• focal myofibrillar damage with focal collagen depositions

thin myocardium ( J:190893 )

• at 8 month

abnormal heart left ventricle morphology ( J:190893 )

• decreased left ventricular posterior wall thickness at diastole and systole

dilated heart left ventricle ( J:190893 )

• at 8 month

dilated heart right ventricle ( J:190893 )

• at 8 month

dilated cardiomyopathy ( J:190893 )

decreased cardiac muscle contractility ( J:190893 )

• contractile dysfunction at 2 months

abnormal vascular endothelial cell physiology ( J:190893 )

• mice exhibit microvascular endothelial activation and increased vascular permeability prior to the development of cardiac fibrosis

increased vascular permeability ( J:190893 )

homeostasis/metabolism

edema ( J:190893 )

• perivascular edema in capillary beds

muscle

abnormal myocardium layer morphology ( J:190893 )

• myocardial fibrosis at 8 month without myocyte cell death

abnormal myocardial fiber morphology ( J:190893 )

• focal myofibrillar damage with focal collagen depositions

thin myocardium ( J:190893 )

• at 8 month

dilated cardiomyopathy ( J:190893 )

decreased cardiac muscle contractility ( J:190893 )

• contractile dysfunction at 2 months

Genotype
MGI:5461514

ht2

• Allelic Composition: Ngf^tm2.1Blhe/Ngf⁺
• Genetic Background: B6.Cg-Ngf^tm2.1Blhe

normal phenotype

no abnormal phenotype detected ( J:190893 )

• mice are used as controls

Genotype
MGI:6388458

ht3

• Allelic Composition: Ngf^tm1(NGF*)Cat/Ngf⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

behavior/neurological

behavior/neurological phenotype ( J:282017 , J:370501 )

N • mice show no learning or memory deficits in the Morris water maze, in the object recognition test, in exploratory activity and preference index (J:282017)

N • mice show normal sociability in the three-chamber sociability test, with a preference for the mouse cage rather than the object as seen in wild-type mice and sociability is unaltered in the social novelty preference test (J:370501)

abnormal touch/nociception ( J:282017 )

• a non-noxious stimulus (small piece of tape applied to the back) takes more time to induce a removal reaction than in controls at 6 months of age, but not at 2 months, indicating that mice are less sensitive to somatosensory inputs

• however, response to gentle stroking of the glabrous skin is normal

increased chemical nociceptive threshold ( J:282017 )

• chemical nociception induced by capsaicin injection in the hindpaw is impaired at 2 months and 6 months of age

increased thermal nociceptive threshold ( J:282017 )

• thermal nociception is normal at 2 month of age but decreases by 6 months of age, with adults showing a higher latency to respond to high-temperature stimulus

• cold sensitivity, measured by topical acetone application on the hindpaw, is reduced at 2 and 6 months of age

integument

abnormal skin sensory neuron innervation pattern ( J:282017 )

• mice show a reduction in hairy and glabrous skin sensory innervation at 6 months of age, but not at 2 months

• however, mice show normal sympathetic innervation of key target organs such as the heart, stomach, kidney, spleen

nervous system

nervous system phenotype ( J:282017 )

N • mice show normal numbers of DRG neurons at P5, 2 months, and 6 months, normal numbers of SCG, normal density of ChAT+ neurons in the medial septum and striatum, normal conduction velocities of the 3 main sensory fiber populations, ABeta, Adelta, and C fibers, and normal Schaffer collateral-CA1 long-term potentiation

abnormal skin sensory neuron innervation pattern ( J:282017 )

• mice show a reduction in hairy and glabrous skin sensory innervation at 6 months of age, but not at 2 months

• however, mice show normal sympathetic innervation of key target organs such as the heart, stomach, kidney, spleen

abnormal sciatic nerve morphology ( J:282017 )

• cross-sectional area of the sciatic nerve is decreased

• sciatic nerve shows a decrease in the number of nonmyelinated axons

• however, the number of myelinated axons in the sciatic nerve is unaffected

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:370501 )

N • mice exhibit normal sweating in the pilocarpine-induced sweat assay

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
hereditary sensory and autonomic neuropathy type 5		DOID:0070145	OMIM:608654	J:282017

Genotype
MGI:6388459

ht4

• Allelic Composition: Ngf^tm1Gne/Ngf⁺
• Genetic Background: involves: 129S7/SvEvBrd

behavior/neurological

abnormal object recognition memory ( J:282017 )

• mice exhibit impaired object recognition memory

• however, exploratory behavior is unaffected

impaired spatial learning ( J:282017 )

• mice exhibit delayed learning in the Morris water maze

nervous system

abnormal cholinergic neuron morphology ( J:282017 )

• density of ChAT+ neurons in the medial septum and striatum are decreased

Genotype
MGI:2175145

ht5

• Allelic Composition: Ngf^tm1Gne/Ngf⁺
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

behavior/neurological

abnormal pain threshold ( J:17792 )

• mutants display a small but significant prolongation in tail flick test

nervous system

dorsal root ganglion hypoplasia ( J:17792 )

• mutants display a decrease in cell number in the dorsal root ganglia

Genotype
MGI:5461512

cx6

• Allelic Composition: Ngf^tm1.1Blhe/Ngf⁺; Ngfr^tm1Jae/Ngfr^tm1Jae
• Genetic Background: B6.Cg-Ngf^tm1.1Blhe Ngfr^tm1Jae

Adult Ngf^tm1.1Blhe/Ngf⁺ mice exhibit dilated cardiomyopathy and fibrosis and this phentoype is rescued in Ngfr^tm1Jae/Ngfr^tm1Jae Ngf^tm1.1Blhe/Ngf⁺ mice

cardiovascular system

cardiovascular system phenotype ( J:190893 )

N • unlike Ngf^tm1.1Blhe heterozygotes, mice do not exhibit ventricular dilation, fibrosis cardiac hypocontractility or premature death

Genotype
MGI:5461513

cx7

• Allelic Composition: Ngf^tm1.1Blhe/Ngf⁺; Sort1^tm1Tew/Sort1^tm1Tew
• Genetic Background: B6.Cg-Ngf^tm1.1Blhe Sort1^tm1Tew

mortality/aging

premature death ( J:190893 )

• most mice die by 4 months

• nearly all mice die by 8 months

cardiovascular system

dilated cardiomyopathy ( J:190893 )

muscle

dilated cardiomyopathy ( J:190893 )

Genotype
MGI:5461511

cx8

• Allelic Composition: Ngf^tm1.1Blhe/Ngf⁺; Tg(Cspg4-DsRed.T1)1Akik/0
• Genetic Background: B6.Cg-Ngf^tm1.1Blhe Tg(Cspg4-DsRed.T1)1Akik

cardiovascular system

abnormal pericyte morphology ( J:190893 )

• shorter pericyte processes with reduced pericyte coverage of the cardiac microvasculature