Phenotypes associated with this allele

• Allele Symbol: Ptk2^tm1Imeg
• Allele Name: targeted mutation 1, Institute of Molecular Embryology and Genetics
• Allele ID: MGI:1857596
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ptk2^tm1Imeg/Ptk2^tm1Imeg	CBA.Cg-Ptk2^tm1Imeg	MGI:3689843
hm2	Ptk2^tm1Imeg/Ptk2^tm1Imeg	involves: C57BL/6 * CBA	MGI:2167575
ht3	Ptk2^tm1Imeg/Ptk2^+	CBA.Cg-Ptk2^tm1Imeg	MGI:3689844

Genotype
MGI:3689843

hm1

• Allelic Composition: Ptk2^tm1Imeg/Ptk2^tm1Imeg
• Genetic Background: CBA.Cg-Ptk2^tm1Imeg

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between somite formation and embryo turning, complete penetrance ( J:82150 )

• die at E8.5

embryo

absent vitelline blood vessels ( J:82150 )

• exhibit absence of vascular structures in the yolk sac

cardiovascular system

abnormal blood vessel morphology ( J:82150 )

• exhibit impaired development of the vasculature system

abnormal vascular endothelial cell development ( J:82150 )

• embryoid bodies differentiating from homozygotes do not develop Pecam1 (CD31)-positive interconnecting vascular channels, indicating inability of endothelial cells to organize into a vascular network, however endothelial cell differentiation is not impaired

absent vitelline blood vessels ( J:82150 )

• exhibit absence of vascular structures in the yolk sac

hemorrhage ( J:82150 )

• 80% exhibit hemorrhage in the placental cone and extraembryonic structures

Genotype
MGI:2167575

hm2

• Allelic Composition: Ptk2^tm1Imeg/Ptk2^tm1Imeg
• Genetic Background: involves: C57BL/6 * CBA

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:29978 )

• lethality between E7.5 and E10.5, with no embryos found at E10.5

embryo

abnormal developmental patterning ( J:29978 )

failure of initiation of embryo turning ( J:29978 )

• none begin to turn and tend to develop flat and crouch at the distal portion of the yolk sac

abnormal ectoderm development ( J:29273 )

abnormal mesoderm development ( J:29273 , J:29978 )

• mesoderm development is poor at E8.5 (J:29273)

• mesoderm is poor in the trunk region (J:29978)

abnormal rostral-caudal axis patterning ( J:29273 , J:29978 )

• anteroposterior axis development is retarded at E8.5 (J:29273)

• rostrocaudal extension is retarded at E8-8.5 (J:29978)

decreased embryo size ( J:29978 )

• embryos tend to be smaller at E7.5

abnormal embryonic tissue morphology ( J:29978 )

abnormal head fold morphology ( J:29978 )

• some embryos do not develop a headfold at E8-8.5 and even if it develops, it is only barely formed

abnormal head mesenchyme morphology ( J:29978 )

• head mesenchyme is poor and compact at E8 and E8.5

abnormal embryonic neuroepithelium morphology ( J:29978 )

• exhibit waving of the neuroectoderm due to insufficient support by poorly developed mesoderm

abnormal notochord morphology ( J:29273 , J:29978 )

• notocord is not present at E8.5 but can been seen in the few most advanced embryos (J:29978)

abnormal somite development ( J:29273 , J:29978 )

• no somites are visible at E8.5, however a few pairs of somatic condensations are visible but show no sign of deepithelialization (J:29978)

abnormal extraembryonic tissue morphology ( J:29978 )

abnormal vitelline artery morphology ( J:29273 )

• omphalomesenteric artery is not apparent at the caudal extremity of the embryo

• omphalomesenteric artery is indistinguishable at E8.5

abnormal allantois morphology ( J:29978 )

• protrusion of allantois is poor

abnormal amnion morphology ( J:29978 )

• amnion is closely apposed to the embryo proper

small amniotic cavity ( J:29978 )

• amniotic cavity is narrow

abnormal visceral yolk sac cavity morphology ( J:29978 )

• blood cells occasionally leak into the yolk sac cavity

small visceral yolk sac ( J:29978 )

• small yolk sac

failure of chorioallantoic fusion ( J:29978 )

• chorioallantoic fusion is not seen

cardiovascular system

abnormal blood vessel morphology ( J:29978 )

• vascularization is poor at E8.5

abnormal dorsal aorta morphology ( J:29273 )

• dorsal aorta is present in the anterior region but is obscured in the caudal region

abnormal vitelline artery morphology ( J:29273 )

• omphalomesenteric artery is not apparent at the caudal extremity of the embryo

• omphalomesenteric artery is indistinguishable at E8.5

abnormal aorta morphology ( J:29978 )

thick myocardium ( J:29978 )

• myocardium is thickened at E8.5

abnormal heart development ( J:29273 , J:29978 )

• cardiac plates fuse at the center to form the heart bulge, but the differentiation of mesocardium and endocardium is not apparent (J:29273)

• show no evident separation of the mesocardium and endocardium at E8.5 (J:29978)

abnormal mesocardium morphology ( J:29273 )

• differentiation of mesocardium and endocardium is not apparent

abnormal endocardium morphology ( J:29273 )

• differentiation of mesocardium and endocardium is not apparent

abnormal blood circulation ( J:29978 )

• no blood cells are found in embryo heart or vasculature at E8.5, though primitive red blood cells do develop in the yolk sac

hemorrhage ( J:29978 )

• blood cells occasionally leak into the yolk sac cavity

cellular

abnormal cell morphology ( J:29273 )

• MEFs from E8 embryos are more rounded and poorly spread when cultured

• MEFs exhibit an increase in the number of focal adhesions

abnormal cell migration ( J:29273 )

• MEFs exhibit reduced cell motility in migration assays with fibronectin as the substratum and do not display a polar migratory conformation but retain a more rounded shape

digestive/alimentary system

abnormal foregut morphology ( J:29978 )

• poor invagination of fore- and hindgut

growth/size/body

decreased embryo size ( J:29978 )

• embryos tend to be smaller at E7.5

abnormal head mesenchyme morphology ( J:29978 )

• head mesenchyme is poor and compact at E8 and E8.5

nervous system

abnormal embryonic neuroepithelium morphology ( J:29978 )

• exhibit waving of the neuroectoderm due to insufficient support by poorly developed mesoderm

muscle

thick myocardium ( J:29978 )

• myocardium is thickened at E8.5

Genotype
MGI:3689844

ht3

• Allelic Composition: Ptk2^tm1Imeg/Ptk2⁺
• Genetic Background: CBA.Cg-Ptk2^tm1Imeg

growth/size/body

decreased embryo size ( J:82150 )

• smaller at E8.5

embryo

decreased embryo size ( J:82150 )

• smaller at E8.5

cardiovascular system

abnormal vascular development ( J:82150 )

• appear slightly delayed in vascular development

abnormal dorsal aorta morphology ( J:82150 )

• do not have fully developed dorsal aortas at E8.5