Phenotypes associated with this allele

• Allele Symbol: Zeb1⁺
• Allele Name: wild type
• Allele ID: MGI:1857516
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Zeb1^Tw/Zeb1^+	B6.Cg-Zeb1^Tw	MGI:5295198
ht2	Zeb1^tm1Yhi/Zeb1^+	involves: 129P2/OlaHsd * C57BL/6	MGI:3812429
ht3	Zeb1^tm2Yhi/Zeb1^+	involves: 129S1/Sv * 129X1/SvJ	MGI:3653687
ht4	Zeb1^Tw/Zeb1^+	involves: C57BL/6J	MGI:2654860
ht5	Zeb1^Tw/Zeb1^+	STOCK PCS	MGI:2654856
cx6	Zeb1^tm2Yhi/Zeb1^+ Zeb2^tm1.2Yhi/Zeb2^+	involves: 129S1/Sv * 129X1/SvJ * ICR	MGI:3653728

Genotype
MGI:5295198

ht1

• Allelic Composition: Zeb1^Tw/Zeb1⁺
• Genetic Background: B6.Cg-Zeb1^Tw

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Inner ear morphology, hearing thresholds, and otoconia of Zeb1^Tw/Zeb1⁺ and Zeb1^Tw/Zeb1^Tw mice

behavior/neurological

increased food intake ( J:177274 )

• starting at 7 weeks of age mice consume 15 - 20% more food

circling ( J:177274 )

growth/size/body

increased body weight ( J:177274 )

obese ( J:177274 )

• in adults

adipose tissue

increased percent body fat/body weight ( J:177274 )

homeostasis/metabolism

abnormal circulating hormone level ( J:177274 )

increased circulating insulin level ( J:177274 )

increased circulating leptin level ( J:177274 )

abnormal circulating lipid level ( J:177274 )

increased circulating corticosterone level ( J:177274 )

increased circulating free fatty acids level ( J:177274 )

increased circulating triglyceride level ( J:177274 )

impaired glucose tolerance ( J:177274 )

• slight glucose intolerance in females at 15 weeks of age

insulin resistance ( J:177274 )

• in females at 15 weeks of age

increased circulating adiponectin level ( J:177274 )

• elevated in the serum

hearing/vestibular/ear

abnormal cochlea morphology ( J:177274 )

• average length of the cochlear duct is 91% that of wild-type controls

abnormal semicircular canal morphology ( J:177274 )

• the contours of the canals are irregular due to small bulges and projections

abnormal lateral semicircular canal morphology ( J:177274 )

• the non-ampullated ends are irregular or constricted

enlarged otoliths ( J:177274 )

• utricles have giant otoconia visible by scanning electron microscopy

increased or absent threshold for auditory brainstem response ( J:177274 )

• increase in the binaural average ABR thresholds at 1 month of age

hematopoietic system

small thymus ( J:177274 )

• in adults

decreased leukocyte cell number ( J:177274 )

• in adults

decreased neutrophil cell number ( J:177274 )

• decrease in the number of polymorphonuclear neutrophils in adults

decreased lymphocyte cell number ( J:177274 )

• in adults

small spleen ( J:177274 )

• in adults

immune system

small thymus ( J:177274 )

• in adults

decreased leukocyte cell number ( J:177274 )

• in adults

decreased neutrophil cell number ( J:177274 )

• decrease in the number of polymorphonuclear neutrophils in adults

decreased lymphocyte cell number ( J:177274 )

• in adults

small spleen ( J:177274 )

• in adults

endocrine/exocrine glands

small thymus ( J:177274 )

• in adults

Genotype
MGI:3812429

ht2

• Allelic Composition: Zeb1^tm1Yhi/Zeb1⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

vision/eye

abnormal iridocorneal angle ( J:135179 )

• iridocorneal adhesions lead to loss of the iridocorneal angle

anterior iris synechia ( J:135179 )

• iridocorneal adhesions are observed in 4 month old mice

• corneolenticular and iridolenticular adhesions are also evident

increased cornea thickness ( J:135179 )

• the cornea of 4 month old mice is more than twice as thick as controls

abnormal cornea stroma morphology ( J:135179 )

• there number of keratocytes found in the cornea are doubled in 4 month old mice compared to age-matched controls

Genotype
MGI:3653687

ht3

• Allelic Composition: Zeb1^tm2Yhi/Zeb1⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

cardiovascular system

abnormal vascular wound healing ( J:110800 )

• exhibit exaggerated neointima formation after artery injury compared to controls

homeostasis/metabolism

abnormal vascular wound healing ( J:110800 )

• exhibit exaggerated neointima formation after artery injury compared to controls

Genotype
MGI:2654860

ht4

• Allelic Composition: Zeb1^Tw/Zeb1⁺
• Genetic Background: involves: C57BL/6J

behavior/neurological

hyperactivity ( J:60496 )

• heterozygotes display hyperactivity, twirling around the cage in periodic spurts

bidirectional circling ( J:60496 )

• heterozygotes exhibit bidirectional "twirling" or circling behavior

growth/size/body

increased susceptibility to age related obesity ( J:60496 )

• most male and female heterozygotes become obese as they reach maturity

• onset of obesity varies from 2 months to 3 months of age in some heterozygotes

decreased body size ( J:60496 )

• young heterozygotes remain smaller than wild-type, despite normal feeding

reproductive system

transmission ratio distortion ( J:60496 )

• a less than 1:1 ratio of heterozygous (43.6%) to wild-type (56.4%) pups is obtained from matings of heterozygous males with wild-type females, suggesting increased lethality either in utero or prior to genotyping at 10 days after birth

female infertility ( J:60496 )

♀ • obese female heterozygotes tend to become sterile

reduced male fertility ( J:60496 )

♂ • obese male heterozygotes breed at a reduced rate

hearing/vestibular/ear

abnormal inner ear morphology ( J:60496 )

• heterozygotes display inner ear defects (not shown)

pigmentation

diluted coat color ( J:60496 )

• most heterozygotes display a lighter coat color than wild-type mice

integument

diluted coat color ( J:60496 )

• most heterozygotes display a lighter coat color than wild-type mice

sparse hair ( J:60496 )

• most heterozygotes have sparse hair

Genotype
MGI:2654856

ht5

• Allelic Composition: Zeb1^Tw/Zeb1⁺
• Genetic Background: STOCK PCS

behavior/neurological

increased aggression towards male mice ( J:274 )

• when allowed to grow up together, 2-month old male heterozygotes often begin to fight to such an extent that they must be either separated or sacrificed

abnormal postural reflex ( J:274 )

• when lifted by the tail, heterozygotes flex their back, tuck their head under, and draw their limbs backwards

• in response to a downward falling movement, heterozygotes fail to thrust their forelimbs forward, indicating absence of a landing reaction

head bobbing ( J:274 )

• heterozygotes display vertical head shaking less often than horizontal head shaking

head shaking ( J:274 )

• heterozygotes display head shaking in a horizontal plane, often combined with circling

circling ( J:274 )

• heterozygotes often exhibit circling combined with horizontal head shaking

abnormal parental behavior ( J:274 )

• some heterozygous parents of both sexes are prone to attacking and killing their young during the first week of life, esp. after being stressed in some way

hearing/vestibular/ear

hearing/vestibular/ear phenotype ( J:274 )

N • heterozygotes are not deaf and generally display a normal cochlea

abnormal scala media morphology ( J:274 )

• 2 of 9 heterozygotes displayed abnormal cochlear duct morphology

• in one of these 2 heterozygotes, the left cochlear duct was normal but the right was hydropic, the organ of Corti was disorganized, and the stria vascularis was missing

• in the other, both cochlear ducts were affected, the apical region was hydropic, the organ of Corti was abnormal, and the stria vascularis was missing form the right ear

abnormal inner ear canal morphology ( J:274 )

• heterozygotes show a highly variable reduction or absence of the horizontal canal along with absence of otoliths and uneven contours of the vertical canals

decreased lateral semicircular canal size ( J:274 )

• at the very least, the horizontal canal is present and only slightly decreased in length, while the otolith number is sometimes reduced

• however, the horizontal canal and ampulla are never entirely absent

abnormal posterior semicircular canal morphology ( J:274 )

• at the very least, the posterior vertical canal displays an abnormal contour

• the uneveness of outline progresses to formation of projections from the walls of the canal

• in severe cases, these projections form branches or duplications running from the canal to the utriculus or the common crus

abnormal semicircular canal ampulla morphology ( J:274 )

• the horizontal ampulla is located more posteriorly than in normal mice

abnormal crista ampullaris morphology ( J:274 )

• the horizontal crista is always abnormal in shape and lies lateral to rather than anterior to the utricle macula

• in contrast, the ampullae and cristae of the vertical canals are normal

abnormal crista ampullaris neuroepithelium morphology ( J:274 )

• the epithelium forming the horizontal crista is folded inwards rather than upwards, forming a pit instead of a ridge in the ventromedial part of the ampulla

abnormal superior semicircular canal morphology ( J:274 )

• at the very least, the anterior vertical canal displays an abnormal contour

• the uneveness of outline progresses to formation of projections from the walls of the canal

• in severe cases, these projections form branches or duplications running from the canal to the utriculus or the common crus

• the arcuate fossa may be variably obliterated by branches from the anterior vertical canal

dilated endolymphatic duct ( J:274 )

• in some ears, the lumen of the endolymphatic ducts is sligtly dilated, suggesting some excess of endolymphatic fluid

abnormal otolith morphology ( J:274 )

• when present, otoliths are often thinner than normal

decreased otolith number ( J:274 )

• a reduction in otolith number is sometimes observed along with a slightly reduced lateral canal

absent otoliths ( J:274 )

• complete loss of otoliths is associated with severe reduction of the lateral canal and increasingly shortened and branched vertical canals

• 9 of 18 heterozygous ears lack both saccular and utricular otoliths, 7 show a normal saccular otolith with a thin utricular otolith in 3 cases, and the remaining 2 ears show a thin saccular otolith only

growth/size/body

increased susceptibility to age related obesity ( J:274 )

• starting at 3 months of age, adult heterozygotes display a tendency to become obese

• however, some heterozygotes exhibit a normal breeding life and remain slim

postnatal growth retardation ( J:274 )

• a few heterozygotes lag behind their wild-type littermates in growth

• however, the growth of young heterozygotes is usually normal

adipose tissue

increased total body fat amount ( J:274 )

• obese heterozygotes display increased deposition of subcutaneous fat in the inguinal and neck regions and between the shoulder blades

abnormal abdominal fat pad morphology ( J:274 )

• obese heterozygotes display increased abdominal fat deposition in the mesentery and around the kidneys, ovaries, and testes

abnormal inguinal fat pad morphology ( J:274 )

• obese heterozygotes display increased deposition of subcutaneous fat in the inguinal region

reproductive system

infertility ( J:274 )

• both sexes are fertile but become sterile if obesity sets in

Genotype
MGI:3653728

cx6

• Allelic Composition: Zeb1^tm2Yhi/Zeb1⁺; Zeb2^tm1.2Yhi/Zeb2⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * ICR

growth/size/body

decreased body size ( J:108819 )

reproductive system

vagina atresia ( J:108819 )

♀ • 70% exhibit closure of the vaginal orifice