All Phenotypes Stat4<tm1Gru> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Stat4^tm1Gru/Stat4^tm1Gru	C.129S2-Stat4^tm1Gru	MGI:3617977
hm2	Stat4^tm1Gru/Stat4^tm1Gru	involves: 129S2/SvPas	MGI:3851529
hm3	Stat4^tm1Gru/Stat4^tm1Gru	involves: 129S2/SvPas * BALB/c	MGI:3617974
hm4	Stat4^tm1Gru/Stat4^tm1Gru	NOD.129S2-Stat4^tm1Gru	MGI:3617975
ht5	Stat4^tm1Gru/Stat4⁺	NOD.129S2-Stat4^tm1Gru	MGI:3617976
ht6	Stat4^ity14/Stat4^tm1Gru	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ	MGI:5578205
cx7	Stat4^tm1Gru/Stat4^tm1Gru Tg(Ins2-NP)25-3Olds/0	involves: 129S2/SvPas * BALB/c * C57BL/6	MGI:3620118
cx8	Stat4^tm1Gru/Stat4^tm1Gru Tg(Ins2-GP)34-20Olds/0	involves: 129S2/SvPas * BALB/c * C57BL/6	MGI:3620122

Allelic Composition	Stat4^tm1Gru/Stat4^tm1Gru
Genetic Background	C.129S2-Stat4^tm1Gru

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4^tm1Gru mutation (4 available); any Stat4 mutation (64 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal CD4-positive T cell differentiation ( J:143729 )

• wild-type CD4⁺ T cells polarized under Th17 conditions can be converted to a Th1-like cells (i.e. they express IFN-gamma) by culturing with IL-12

• mutant polarized Th17 CD4⁺ T cells fail to convert to the Th1-like state in the presence of IL-12

decreased circulating interferon-gamma level ( J:90600 )

• reduction in the serum level of IFNG are seen compared to levels in diabetic and non-diabetic NOD mice, but serum level is comparable to that in Stat4-null NOD mice

decreased circulating interleukin-2 level ( J:90600 )

• reduction in the serum level of IL-2 is seen compared to level in diabetic and non-diabetic NOD mice, but serum level of IL-12 is comparable to that of Stat4-null NOD mice

homeostasis/metabolism

decreased circulating interferon-gamma level ( J:90600 )

• reduction in the serum level of IFNG are seen compared to levels in diabetic and non-diabetic NOD mice, but serum level is comparable to that in Stat4-null NOD mice

decreased circulating interleukin-2 level ( J:90600 )

• reduction in the serum level of IL-2 is seen compared to level in diabetic and non-diabetic NOD mice, but serum level of IL-12 is comparable to that of Stat4-null NOD mice

hematopoietic system

abnormal CD4-positive T cell differentiation ( J:143729 )

• wild-type CD4⁺ T cells polarized under Th17 conditions can be converted to a Th1-like cells (i.e. they express IFN-gamma) by culturing with IL-12

• mutant polarized Th17 CD4⁺ T cells fail to convert to the Th1-like state in the presence of IL-12

renal/urinary system

abnormal kidney physiology ( J:84825 )

• onset of ischemia effects is delayed

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:106340

Allelic Composition	Stat4^tm1Gru/Stat4^tm1Gru
Genetic Background	involves: 129S2/SvPas

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4^tm1Gru mutation (4 available); any Stat4 mutation (64 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal cytokine secretion ( J:112600 )

• T cells from mice immunized with keyhole limpet hemocyanin (KLH) in CFA (conjugated Freund's adjuvant) produce less IL-4 and interferon gamma than wild-type in response to KLH restimulation; IL-17a levels are comparable to controls

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

increased susceptibility to bacterial infection induced morbidity/mortality ( J:163843 )

• Salmonella-infected mice exhibit increased mortality compared with similarly treated heterozygous or wild-type mice

immune system

abnormal lymphocyte physiology ( J:34059 )

abnormal T cell proliferation ( J:34059 )

• activated T lymphocytes stimulated with IL-12 for 48 hours do not proliferate, whereas activated lymphocytes from control mice exhibit dose-dependent proliferation

abnormal NK cell physiology ( J:34059 )

• IL-12 stimulated NK cells do not show enhanced cytotoxicity compared to unstimulated cells, while stimulated control cells show a significant enhancement (30% specific lysis compared to 10% in unstimulated control cells)

abnormal T-helper 1 physiology ( J:34059 )

• cultured lymphocytes differentiated into Th1 cells by anti-CD3 or heat-killed Listeria monocytogenes produce less than 20% of the IFNG produced by control cells; lymphotoxin production also is significantly reduced compared to control cells

• significant levels of Th2 cytokines IL-5 and IL-10 are detected in supernatants from these cultured lymphocytes

abnormal T-helper 2 physiology ( J:34059 )

• lymphocytes cultured in the presence of IL-4 and IFNG produce significantly higher levels of Th2 cytokines than control cells

decreased circulating interferon-gamma level ( J:163843 )

• in Salmonella-infected mice

decreased interferon-gamma secretion ( J:34059 )

• following Il-2 stimulation for 24 hours, lymphocytes from mutants do not show increased IFNG levels compared to a 15-fold increase seen in activated cells from control mice

increased susceptibility to bacterial infection ( J:163843 )

• Salmonella-infected mice exhibit increased mortality, increased organ bacterial burden, and decreased IFN-gamma levels compared with similarly treated heterozygous or wild-type mice

increased susceptibility to bacterial infection induced morbidity/mortality ( J:163843 )

• Salmonella-infected mice exhibit increased mortality compared with similarly treated heterozygous or wild-type mice

hematopoietic system

abnormal lymphocyte physiology ( J:34059 )

abnormal T cell proliferation ( J:34059 )

• activated T lymphocytes stimulated with IL-12 for 48 hours do not proliferate, whereas activated lymphocytes from control mice exhibit dose-dependent proliferation

abnormal NK cell physiology ( J:34059 )

abnormal T-helper 1 physiology ( J:34059 )

• significant levels of Th2 cytokines IL-5 and IL-10 are detected in supernatants from these cultured lymphocytes

abnormal T-helper 2 physiology ( J:34059 )

• lymphocytes cultured in the presence of IL-4 and IFNG produce significantly higher levels of Th2 cytokines than control cells

homeostasis/metabolism

decreased circulating interferon-gamma level ( J:163843 )

• in Salmonella-infected mice

cellular

abnormal T cell proliferation ( J:34059 )

• activated T lymphocytes stimulated with IL-12 for 48 hours do not proliferate, whereas activated lymphocytes from control mice exhibit dose-dependent proliferation

Allelic Composition	Stat4^tm1Gru/Stat4^tm1Gru
Genetic Background	NOD.129S2-Stat4^tm1Gru

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4^tm1Gru mutation (4 available); any Stat4 mutation (64 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased circulating interferon-gamma level ( J:90600 )

• reduction in the serum level of IFNG is seen compared to diabetic and non-diabetic NOD mice

decreased circulating interleukin-2 level ( J:90600 )

• a reduction in the serum level of IL-2 is observed compared to diabetic and non-diabetic NOD mice

decreased susceptibility to autoimmune diabetes ( J:90600 )

• no female mutants develop diabetes (blood glucose levels above 13.9 mmol/l for 3 readings) over a 10-month period compared with 74% of female wild-type Stat4 NOD mice, which is similar to female NOD mice (80%)

abnormal inflammatory response ( J:90600 )

• noc cellular infiltration is observed at 40 weeks of age compared to massive and invasive cellular infiltration seen in diabetic NOD controls and heterozygous Stat4 NOD or wild-type Stat4 mice

• a significant reduction in the numbers of CD4+ and CD8+ cells in islets is observed compared to diabetic NOD mice

endocrine/exocrine glands

abnormal pancreatic beta cell morphology ( J:90600 )

• well-preserved insulin-positive cells are seen in the islets of mutants whereas such cells are rare in Stat4 heterozygous and wild-type Stat4 NOD mice

abnormal insulin secretion ( J:90600 )

• compared to NOD controls, islets in Stat4 null NOD mice show well preserved insulin secretion at basal glucose levels and in response to high glucose

homeostasis/metabolism

abnormal insulin secretion ( J:90600 )

• compared to NOD controls, islets in Stat4 null NOD mice show well preserved insulin secretion at basal glucose levels and in response to high glucose

decreased circulating interferon-gamma level ( J:90600 )

• reduction in the serum level of IFNG is seen compared to diabetic and non-diabetic NOD mice

decreased circulating interleukin-2 level ( J:90600 )

• a reduction in the serum level of IL-2 is observed compared to diabetic and non-diabetic NOD mice

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
NOT	type 1 diabetes mellitus	DOID:9744	OMIM:222100	J:90600

Allelic Composition	Stat4^tm1Gru/Stat4⁺
Genetic Background	NOD.129S2-Stat4^tm1Gru

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4^tm1Gru mutation (4 available); any Stat4 mutation (64 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

homeostasis/metabolism

abnormal circulating glucose level ( J:90600 )

• a significant decrease in the incidence of diabetes (30%) is observed compared with wild-type Stat4 NOD mice (74%); diabetes is diagnosed when 3 blood glucose measures are equal to or higher than 13.9 mmol/l

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:90600

Allelic Composition	Stat4^ity14/Stat4^tm1Gru
Genetic Background	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4^ity14 mutation (0 available); any Stat4 mutation (64 available)
Stat4^tm1Gru mutation (4 available); any Stat4 mutation (64 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased susceptibility to bacterial infection induced morbidity/mortality ( J:207092 )

• mice infected with Salmonella Typhimurium exhibit increased lethality compared with wild-type mice

mortality/aging

increased susceptibility to bacterial infection induced morbidity/mortality ( J:207092 )

• mice infected with Salmonella Typhimurium exhibit increased lethality compared with wild-type mice

Allelic Composition	Stat4^tm1Gru/Stat4^tm1Gru Tg(Ins2-NP)25-3Olds/0
Genetic Background	involves: 129S2/SvPas * BALB/c * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4^tm1Gru mutation (4 available); any Stat4 mutation (64 available)
Tg(Ins2-NP)25-3Olds mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

insulitis ( J:107047 )

• mutants have a lower degree of insulitis

abnormal cytotoxic T cell physiology ( J:107047 )

• a 5-fold reduction in LCMV-specific CTL precursors is seen after viral challenge with LCMV

decreased interferon-gamma secretion ( J:107047 )

• splenocytes are unresponsive to IL-12 stimulation and do not produce significant levels oi IFNgamma

decreased susceptibility to autoimmune diabetes ( J:107047 )

• a significant reduction in CD4+ T cell-dependent (2 consecutive measures of blood glucose >250 mg/dl) diabetes incidence is observed in mutants (30%) versus controls (85%) when infected with LCMV to induce IDDM

endocrine/exocrine glands

insulitis ( J:107047 )

• mutants have a lower degree of insulitis

hematopoietic system

abnormal cytotoxic T cell physiology ( J:107047 )

• a 5-fold reduction in LCMV-specific CTL precursors is seen after viral challenge with LCMV

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:107047

Allelic Composition	Stat4^tm1Gru/Stat4^tm1Gru Tg(Ins2-GP)34-20Olds/0
Genetic Background	involves: 129S2/SvPas * BALB/c * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4^tm1Gru mutation (4 available); any Stat4 mutation (64 available)
Tg(Ins2-GP)34-20Olds mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal susceptibility to autoimmune disorder ( J:107047 )

• Stat4 deficiency does not affect the incidence of CD4+ T cell independent diabetes

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:107047

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/16/2024 MGI 6.23