Phenotypes associated with this allele

• Allele Symbol: Igll1^tm1Cgn
• Allele Name: targeted mutation 1, University of Cologne
• Allele ID: MGI:1857189
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Igll1^tm1Cgn/Igll1^tm1Cgn	involves: 129S2/SvPas * C57BL/6	MGI:2179332
cx2	Igk^tm1Rsky/Igk^+ Igll1^tm1Cgn/Igll1^tm1Cgn	involves: 129P2/OlaHsd * 129S2/SvPas * BALB/c * C57BL/6	MGI:3689735
cx3	Igll1^tm1Cgn/Igll1^tm1Cgn Tg(Igk-V4CH27)1-2Shc/0	involves: 129S2/SvPas * BALB/c * C57BL/6 * SJL	MGI:3689734

Genotype
MGI:2179332

hm1

• Allelic Composition: Igll1^tm1Cgn/Igll1^tm1Cgn
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal lymphopoiesis ( J:64284 )

increased pro-B cell number ( J:64284 )

• about a 2-fold increase compared to wild-type

abnormal B cell morphology ( J:64284 )

• in young (6 weeks of age) but not old (5 - 6 months of age) mice B cells are larger and appear to mostly be activated

• however, cells that do mature display normal allelic exclusion

decreased B cell number ( J:35357 , J:64284 )

• in the spleen at 6 - 12 weeks of age, B cell numbers are about 1/3 that of wild-type mice (J:35357)

• at 5-6 weeks of age in the peripheral blood; T cell numbers are similar to wild-type (J:64284)

• at 5-6 weeks of age the ratio of B to T cells in the blood is decreased about 25- to 65-fold relative to wild-type mice and the number of splenic B cells is reduced about 10- to 30-fold (J:64284)

• at 4 months of age, B cells numbers in the blood and spleen are reduced 5- to 13-fold and 3- to 5-fold, respectively (J:64284)

abnormal B-1 B cell number ( J:64284 )

• at 2 weeks of age the number of lymphocytes in the peritoneum is reduced; however, at 5-7 weeks of age the number of B-1 cells in the peritoneum is similar to or increased compared to wild-type mice

abnormal pre-B cell morphology ( J:64284 )

• about a 2-fold increase in large early pre-B cells and a 40-fold decrease in small pre-B cells compared to wild-type

spleen hypoplasia ( J:64284 )

• spleen cellularity is reduced about 4-fold and 2-fold at 2 weeks and 5-6 weeks of age, respectively

decreased IgG level ( J:64284 )

• decreased levels of IgG3, IgG2b, and, to a lesser degree, IgG1 in response to a T-cell dependent antigen with overall antibody response reduced about 5- to 10-fold

decreased IgG1 level ( J:64284 )

decreased IgG2b level ( J:64284 )

decreased IgG3 level ( J:64284 )

hematopoietic system

abnormal lymphopoiesis ( J:64284 )

increased pro-B cell number ( J:64284 )

• about a 2-fold increase compared to wild-type

abnormal B cell morphology ( J:64284 )

• in young (6 weeks of age) but not old (5 - 6 months of age) mice B cells are larger and appear to mostly be activated

• however, cells that do mature display normal allelic exclusion

decreased B cell number ( J:35357 , J:64284 )

• in the spleen at 6 - 12 weeks of age, B cell numbers are about 1/3 that of wild-type mice (J:35357)

• at 5-6 weeks of age in the peripheral blood; T cell numbers are similar to wild-type (J:64284)

• at 5-6 weeks of age the ratio of B to T cells in the blood is decreased about 25- to 65-fold relative to wild-type mice and the number of splenic B cells is reduced about 10- to 30-fold (J:64284)

• at 4 months of age, B cells numbers in the blood and spleen are reduced 5- to 13-fold and 3- to 5-fold, respectively (J:64284)

abnormal B-1 B cell number ( J:64284 )

• at 2 weeks of age the number of lymphocytes in the peritoneum is reduced; however, at 5-7 weeks of age the number of B-1 cells in the peritoneum is similar to or increased compared to wild-type mice

abnormal pre-B cell morphology ( J:64284 )

• about a 2-fold increase in large early pre-B cells and a 40-fold decrease in small pre-B cells compared to wild-type

spleen hypoplasia ( J:64284 )

• spleen cellularity is reduced about 4-fold and 2-fold at 2 weeks and 5-6 weeks of age, respectively

decreased IgG level ( J:64284 )

• decreased levels of IgG3, IgG2b, and, to a lesser degree, IgG1 in response to a T-cell dependent antigen with overall antibody response reduced about 5- to 10-fold

decreased IgG1 level ( J:64284 )

decreased IgG2b level ( J:64284 )

decreased IgG3 level ( J:64284 )

Genotype
MGI:3689735

cx2

• Allelic Composition: Igk^tm1Rsky/Igk⁺; Igll1^tm1Cgn/Igll1^tm1Cgn
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * BALB/c * C57BL/6

immune system

abnormal B cell differentiation ( J:35357 )

• only 5.5% of CD43+ B cell progenitors express the kappa chain in the cytoplasm compared to 22% of wild-type cells at a similar developmental stage which express a kappa chain

decreased immature B cell number ( J:35357 )

• about a 5-fold reduction in immature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus

decreased B cell number ( J:35357 )

• about a 5-fold reduction in mature and immature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus

• in the spleen at 6 - 12 weeks of age, B cell numbers are about 1/3 that of wild-type mice

decreased mature B cell number ( J:35357 )

• about a 5-fold reduction in mature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus

decreased pre-B cell number ( J:35357 )

• about a 3-fold reduction compared to wild-type mice

hematopoietic system

abnormal B cell differentiation ( J:35357 )

• only 5.5% of CD43+ B cell progenitors express the kappa chain in the cytoplasm compared to 22% of wild-type cells at a similar developmental stage which express a kappa chain

decreased immature B cell number ( J:35357 )

• about a 5-fold reduction in immature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus

decreased B cell number ( J:35357 )

• about a 5-fold reduction in mature and immature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus

• in the spleen at 6 - 12 weeks of age, B cell numbers are about 1/3 that of wild-type mice

decreased mature B cell number ( J:35357 )

• about a 5-fold reduction in mature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus

decreased pre-B cell number ( J:35357 )

• about a 3-fold reduction compared to wild-type mice

Genotype
MGI:3689734

cx3

• Allelic Composition: Igll1^tm1Cgn/Igll1^tm1Cgn; Tg(Igk-V4CH27)1-2Shc/0
• Genetic Background: involves: 129S2/SvPas * BALB/c * C57BL/6 * SJL

immune system

abnormal B cell differentiation ( J:35357 )

• more than 80% of CD43+ B cell progenitors express the transgene in the cytoplasm, compared to only 22% of wild-type cells at a similar developmental stage which express a kappa chain

decreased pre-B cell number ( J:35357 )

• about a 3- to 4-fold reduction in pre-B cells; however, the overall numbers of immature and mature B cell numbers are similar to wild-type

hematopoietic system

abnormal B cell differentiation ( J:35357 )

• more than 80% of CD43+ B cell progenitors express the transgene in the cytoplasm, compared to only 22% of wild-type cells at a similar developmental stage which express a kappa chain

decreased pre-B cell number ( J:35357 )

• about a 3- to 4-fold reduction in pre-B cells; however, the overall numbers of immature and mature B cell numbers are similar to wild-type