All Phenotypes Kitl<Sl-gb> MGI Mouse

lethality throughout fetal growth and development, incomplete penetrance ( J:79293 )

perinatal lethality, complete penetrance ( J:79293 )

• some are born live but all die by P2

prenatal lethality, incomplete penetrance ( J:79293 )

abnormal primordial germ cell morphology ( J:115437 )

• between E9.5 and 10.5, most PGCs are found with in the hindgut and these have abnormal morphology, while in wild-type embryos most PGCs are found in dorsal portions of mesentery

decreased primordial germ cell number ( J:115437 )

• at E9.5, PGCs are located primarily in the ventral axis of the hindgut while in wild-type PGCs are found primarily associated with the hindgut epithelium or in the dorsal axis of the hindgut; total PGC number in mutant embryos is 19% of wild-type

• at E10.5, mutants have 4% of the wild-type number of PGCs

absent primordial germ cells ( J:115437 )

• at E11.5, no alkaline phosphatase-positive primordial germ cells (PGCs) are detected in genital ridges

increased primordial germ cell apoptosis ( J:115437 )

• at E10.5, many PGCs in hindgut appear to be disintegrating; abnormal PGCs in hindgut tend to be nonmotile and apoptotic

abnormal primordial germ cell migration ( J:115437 )

• at E10.5, only 31% of total PGCs have migrated from hindgut, compared to 93% in wild-type

abnormal primordial germ cell proliferation ( J:115437 )

• at E10.5, migratory PGCs in the hindgut are observed to proliferate

macrocytic anemia ( J:79293 )

• severe at birth

low mean erythrocyte cell number ( J:79293 )

• significantly lower than wild-type at P1 (RBC count is 17% of wild-type value)