Dnajb7^em1Qyue
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnajb7^em1Qyue
• Name: DnaJ heat shock protein family (Hsp40) member B7; endonuclease-mediated mutation 1, Qiuling Yue
• MGI ID: MGI:8461481
• Gene: Dnajb7 Location: Chr15:81291289-81292474 bp, - strand Genetic Position: Chr15, 38.09 cM
• Alliance: Dnajb7^em1Qyue page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details:

  CRISPR/Cas9-mediated recombination targeting the exon containing the J-domain using sgRNAs 5'-ACTGTTTAAAAGGCCCTCGA-3' and 5'-TGCCACACTATTTACAAGAA-3' generated a 335 bp deletion, resulting in a frameshift and premature stop codon. In situ hybridization showed absent mRNA in testes and Western blot analysis confirmed absence of protein in testes. (J:344341)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnajb7 Mutation: 14 strains or lines available

References

• Original: J:344341 Bai S, et al., DNAJB7 is dispensable for male fertility in mice. Reprod Biol Endocrinol. 2023 Mar 31;21(1):32
• All: 1 reference(s)