Hs3st3a1^em1Jiliu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hs3st3a1^em1Jiliu
• Name: heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1; endonuclease-mediated mutation 1, Jian Liu
• MGI ID: MGI:8460917
• Gene: Hs3st3a1 Location: Chr11:64326158-64413667 bp, + strand Genetic Position: Chr11, 40.24 cM
• Alliance: Hs3st3a1^em1Jiliu page

Mutation origin

• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details:

  A zinc finger nuclease (ZFN) mRNA construct was used to generate a targeted deletion in exon 1 of Hs3st3a1 gene. The ZFN mRNAs were microinjected into single-cell Hs3st3b1+/ mouse embryos. Mutant mice were confirmed to have cosegregation of both the newly targeted Hs3st3a gene and the previous Hs3st3b knockout deletion on the same chromosome. Three founders were selected with deletion of both Hs3st3a1 and Hs3st3b1 segregated on the same allele. Founders B9, B14 and D6 had 10, 26 and 22 base pair deletions, respectively, in the gene. The lines were not distinguished in experiments. (J:360574, J:353621)

Expression

In Mice Carrying this Mutation:

224 assay results 3 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hs3st3a1 Mutation: 11 strains or lines available

References

• Original: J:360574 Wang Z, et al., Increased 3-O-sulfated heparan sulfate in Alzheimer's disease brain is associated with genetic risk gene HS3ST1. Sci Adv. 2023 May 26;9(21):eadf6232
• All: 3 reference(s)