Igh^tm15.1Fwa
Targeted Allele Detail

Summary

• Symbol: Igh^tm15.1Fwa
• Name: immunoglobulin heavy chain complex; targeted mutation 15.1, Frederick W Alt
• MGI ID: MGI:8394053
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:389722
• Parent Cell Line: EF1 (ES Cell)
• Strain of Origin: 129S/SvEv x C57BL/6

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details:

  The mouse heavy (Igh) immunoglobulin chain regions were modified to incorporate human sequences to enhance the testing of broadly neutralizing antibody (bnAb) responses to conserved HIV-1 envelope protein immunogens. The mouse Igh region on Chromosome 12: the mouse VH5-2 (Ighv5-2) segment was replaced with human VH (IGHV3-33*05); the mouse intergenic control region 1 (IGCR1, located between the VH and D clusters) was deleted; and all four of the mouse JH (mJH) segments were replaced with a single human JH (IGHJ6*03). A human D segment (IGHD3-3*01) was placed immediately upstream of the hJH, in place of mouse DQ52. LoxP-flanked neomycin cassettes in each of the targeting constructs were removed from ES cell clones through the transient expression of Adeno-cre recombinase. (J:101977, J:389722)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 54 strains or lines available

References

• Original: J:389722 Tian M, et al., Mouse models with human antibody repertoires for inducing multiple lineages of HIV-1 broadly neutralizing antibodies. bioRxiv. 2026 June 30
• All: 2 reference(s)