Foxl2^em1(cre)Hy
Endonuclease-mediated Allele Detail

Summary

• Symbol: Foxl2^em1(cre)Hy
• Name: forkhead box L2; endonuclease-mediated mutation 1, Humphrey Hung-Chang Yao
• MGI ID: MGI:8363195
• Synonyms: Foxl2^Cre
• Gene: Foxl2 Location: Chr9:98837495-98840601 bp, + strand Genetic Position: Chr9, 51.41 cM, cytoband E4
• Alliance: Foxl2^em1(cre)Hy page

Mutation origin

• Strain of Origin: B6;129P2-Foxl2^{tm1(GFP/cre/ERT2)Pzg}/J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout, Recombinase)
• Mutation: Insertion
• Foxl2^em1(cre)Hy expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Foxl2 (MGI:1349428)

• Mutation details:

  Using CRISPR/Cas9 technology, (sgRNA) targeting specifically the 5' end of the ERT2 domain: GGCGATCTCGAGC- CATCTGCNGG[PAM] was used to modify the Foxl2^{tm1(GFP/cre/ERT2)Pzg} allele to delete single nucleotide that resulted in a premature stop codon eight amino acids after the CRE ORF (novel C-terminus CRE tail: LEPLLET) sequence. The resulting allele contains a non-functional GFP and constitutive Cre and an FRT site flanked Kanamycin/neomycin selection cassette disrupting the exon containing the ATG translation start-site. (J:101977, J:388049)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Foxl2 (mouse) Summary of all recombinase alleles driven by Foxl2. Alliance mouse Foxl2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Foxl2 Mutation: 18 strains or lines available

References

• Original: J:388049 Nicol B, et al., A New Constitutive Foxl2-Cre Mouse Model Reveals Foxl2-Derived Cell Fate in Reproductive and Non-Reproductive Tissues. Biol Reprod. 2026 Jun 3
• All: 2 reference(s)