Mga^em1Okud
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mga^em1Okud
• Name: MAX gene associated; endonuclease-mediated mutation 1, Akihiko Okuda
• MGI ID: MGI:8347574
• Synonyms: Mga deltaExon19a
• Gene: Mga Location: Chr2:119727709-119800062 bp, + strand Genetic Position: Chr2, 59.97 cM
• Alliance: Mga^em1Okud page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas-mediated targeting deleted exon 19a. The resulting allele fails to produce a germ cell-specific isoform. RT-PCR analysis using primers designed to distinguish between canonical and splice variant mRNAs confirmed the absence of the larger PCR product derived from the splice variant mRNA (which includes exon 19a) in the testes of homozygous mutant males. (J:386064)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mga Mutation: 145 strains or lines available

References

• Original: J:386064 Kitamura Y, et al., Alternative splicing for germ cell-specific Mga transcript can be eliminated without compromising mouse viability or fertility. Dev Growth Differ. 2022 Sep;64(7):409-416
• All: 1 reference(s)