Gt(ROSA)26Sor^{tm1(CAG-Mfge8*D89E)Ksawa}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Mfge8*D89E)Ksawa}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Kazunobu Sawamoto
• MGI ID: MGI:8347539
• Synonyms: R26-D89E
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Mfge8*D89E)Ksawa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:329277
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-Mfge8*D89E)Ksawa} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mfge8 (MGI:102768)	*D89E

• Mutation details: The targeting construct introduced a CAG promoter, neomycin resistance cassette, SV40 STOP sequence, FRT site, IRES, EGFP reporter gene, SV40 Poly(A) signal, and mouse gene encoding the amino acid substitution aspartic acid with glutamic acid at position 89 (D89E). (J:329277)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1212 strains or lines available

References

• Original: J:329277 Kurematsu C, et al., Synaptic pruning of murine adult-born neurons by microglia depends on phosphatidylserine. J Exp Med. 2022 Apr 4;219(4):e20202304
• All: 1 reference(s)