Gt(ROSA)26Sor^{em28.1(CAG-Akaluc*)Rbrc}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em28.1(CAG-Akaluc*)Rbrc}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 28.1, RIKEN BioResource Center
• MGI ID: MGI:8347525
• Synonyms: CAG-ATG out-DIO-Akaluc*, Gt(ROSA)26Sor^{em28.1(CAG-DIO-Akaluc*)Rbrc}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em28.1(CAG-Akaluc*)Rbrc} page

Mutation origin

• Strain of Origin: C57BL/6NJcl

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: This knockin allele carries a CAG-ATG out-DIO-Akaluc* construct at the ROSA26 locus. A CAG-ATG out-DIO-Akaluc* is composed of ATG-less Akaluc coding seqence placed in an inverted orientation relative to CAG promoter between the DIO (loxP and lox2272-based) cassette. Kozak sequence and translation initiation codon are placed upstream of 5' DIO cassette. Cre-mediated recombination restored proper orientation for expression. (J:385571)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1212 strains or lines available

References

• Original: J:385571 Nakashiba T, et al., Assessing leaky expression of Cre-dependent DNA constructs in the mouse genome using sensitive bioluminescent reporters. Cell Rep Methods. 2026 Apr 20;6(4):101369
• All: 1 reference(s)