Gt(ROSA)26Sor^{tm1(CAG-mKOk/atpc*/mEGFP)Ymto}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-mKOk/atpc*/mEGFP)Ymto}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Masamichi Yamamoto
• MGI ID: MGI:8297342
• Synonyms: AVID
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-mKOk/atpc*/mEGFP)Ymto} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:381428
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details:

  The targeting vector was created to insert a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a floxed cassette containing STOP-FRT-PGK-Neo-BGH polyA-FRT-STOP, the GO-ATeam2 sequence, and a poly A signal. Specifically, the GO-ATeam2 sequence consists of three fused components: an orange fluorescent protein (mKusabira-Orange-kappa; mKOk), the epsilon subunit of FoF1-ATP synthase gene (atpC) derived from B subtilis, and a circularly permuted monomeric enhanced green fluorescent protein (mEGFP; cp173-mEGFP). The vector was targeted to the Gt(ROSA)26Sor locus. (J:101977, J:381428)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1243 strains or lines available

References

• Original: J:381428 Ohnishi Y, et al., AVID mouse: A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice. Cell Rep. 2025 Sep 23;44(9):116246
• All: 2 reference(s)