Morc2a^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Morc2a^{tm1d(KOMP)Wtsi}
• Name: microrchidia 2A; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:8296691
• Gene: Morc2a Location: Chr11:3599191-3640477 bp, + strand Genetic Position: Chr11, 2.56 cM
• Alliance: Morc2a^{tm1d(KOMP)Wtsi} page
• IMPC: Morc2a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:377141
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 3611392 of Chromosome 11 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 3612119. Exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:377141)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Morc2a Mutation: 59 strains or lines available

References

• Original: J:377141 Kang Z, et al., Stage-dependent dual mechanisms of MORC2 in retrotransposon silencing and sex chromosome inactivation in germ cells. Nucleic Acids Res. 2025 Nov 26;53(22)
• All: 1 reference(s)