Lhcgr^em1Cmgm
Endonuclease-mediated Allele Detail

Summary

• Symbol: Lhcgr^em1Cmgm
• Name: luteinizing hormone/choriogonadotropin receptor; endonuclease-mediated mutation 1, Siu-Pok Yee
• MGI ID: MGI:8296179
• Synonyms: Lhr-COIN
• Gene: Lhcgr Location: Chr17:89023909-89099418 bp, - strand Genetic Position: Chr17, 58.35 cM
• Alliance: Lhcgr^em1Cmgm page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details:

  Using CRISPR/Cas9 endonuclease-mediated genome editing. A single guide RNA (5'-GCAAAGGACGGGCAAGCGGA) was used to introduce a conditional by inversion (COIN) cassette into intron 1 of the luteinizing hormone/choriogonadotropin receptor (Lhcgr) gene. The entire construct has an initial inward facing lox2722 site, an inward facing lox5171 site, the inverted cassette containing a rabbit beta-globin splice acceptor (SA), a P2A self-cleaving peptide, an enhanced green fluorescent protein gene (EGFP), and a bovine growth hormone (bGH) polyadenylation signal. The inversion cassette is then followed by lox2272 and lox5171 sites, both in reverse orientation. (J:94077, J:388887)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lhcgr Mutation: 45 strains or lines available

References

• Original: J:388887 Owen CM, et al., Conditional replacement of the mouse luteinizing hormone receptor with green fluorescent protein, enabling imaging of cell migration during ovulation. Endocrinology. 2026 May 26;167(7)
• All: 2 reference(s)