Gt(ROSA)26Sor^{em1(CAG-DHBmClover3,-H2BmRuby3)Nihsr}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-DHBmClover3,-H2BmRuby3)Nihsr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Sergio Ruiz Macias
• MGI ID: MGI:8292100
• Synonyms: ROSA26-DHB/H2B
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-DHBmClover3,-H2BmRuby3)Nihsr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:366315
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: This allele contains a DHB-Clover3-2A-H2B-Ruby3 transgene, a fluorescent reporter of CDK activity, inserted into the locus via CRISPR-targeting. Expression is driven by a chimeric CMV enhancer/chicken beta-actin promoter. (J:366315)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1210 strains or lines available

References

• Original: J:366315 Saykali B, et al., Lineage-specific CDK activity dynamics characterize early mammalian development. Cell Rep. 2025 Apr 22;44(4):115558
• All: 2 reference(s)