Nectin1^em2Yutom
Endonuclease-mediated Allele Detail

Summary

• Symbol: Nectin1^em2Yutom
• Name: nectin cell adhesion molecule 1; endonuclease-mediated mutation 2, Yukiko Tomioka
• MGI ID: MGI:8286430
• Synonyms: nectin-1 135 KO
• Gene: Nectin1 Location: Chr9:43655251-43718758 bp, + strand Genetic Position: Chr9, 24.42 cM, cytoband B
• Alliance: Nectin1^em2Yutom page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Phenylalanine codon 129 (TTC) was targeted for change to alanine (GCC) (p.F129A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology, resulting in an unintended 16 bp deletion (CACCTTCCCTACGGGC) that leads to a frameshift and premature stop codon (p.F129Vfs*9). The mutation protects the mice from lethal pseudorabies virus (PRV) infections in a virus-titer-dependent manner. (J:355676)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nectin1 Mutation: 22 strains or lines available

References

• Original: J:355676 Tomioka Y, et al., Single amino acid mutation of nectin-1 provides remarkable resistance against lethal pseudorabies virus infection in mice. J Vet Med Sci. 2024 Jan 26;86(1):120-127
• All: 1 reference(s)