Sp1^{em1(pb/ERT2)Jdd}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sp1^{em1(pb/ERT2)Jdd}
• Name: trans-acting transcription factor 1; endonuclease-mediated mutation 1, Joseph D Dougherty
• MGI ID: MGI:8282706
• Synonyms: Sp1-iCC
• Gene: Sp1 Location: Chr15:102314751-102344839 bp, + strand Genetic Position: Chr15, 57.55 cM
• Alliance: Sp1^{em1(pb/ERT2)Jdd} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Inducible, Transposase)
• Inducer: tamoxifen
• Mutation: Insertion
• Mutation details: Using CRISPR/Cas9 endonuclease-mediated genome editing, a single guide RNA was used to introduce a hyperactive piggyBac transposase (hyPB), myc tag and an ERT2 fusion gene and an 18aa linker sequence (5'- CAAGTGGGCGGCGGCGCGCCCCGCCTGGGCGGCGGACCCAAGC-3') into the C-terminal domain of the trans-acting transcription factor 1 (Sp1) gene. (J:94077, J:378521)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sp1 Mutation: 166 strains or lines available

References

• Original: J:378521 Sarafinovska S, et al., Inducible Calling Cards: Developing Mouse Reagents for Experimentally Controlled Transposon Insertion in vivo. bioRxiv. 2025
• All: 2 reference(s)