Ezh2^em1Kaig
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ezh2^em1Kaig
• Name: enhancer of zeste 2 polycomb repressive complex 2 subunit; endonuclease-mediated mutation 1, Kai Ge
• MGI ID: MGI:8282418
• Synonyms: Ezh2(F6671)
• Gene: Ezh2 Location: Chr6:47507073-47572275 bp, - strand Genetic Position: Chr6, 22.92 cM, cytoband B
• Alliance: Ezh2^em1Kaig page

Mutation origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1/J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details:

  The allele was generated using CRISPR/Cpf1(Cas12a) endonuclease-mediated genome editing. A single guide RNA (5'-TGTTCAACTTGAACAATGGTATGT-3') was used to insert point mutations resulting in a phenylalanine to isoleucine amino acid replacement (F667I) in the SET domain of the Ezh2 gene. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ezh2 Mutation: 72 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)