Ctnna1^em1Cgot
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ctnna1^em1Cgot
• Name: catenin alpha 1; endonuclease-mediated mutation 1, Cara Gottardi
• MGI ID: MGI:8270339
• Gene: Ctnna1 Location: Chr18:35251955-35387829 bp, + strand Genetic Position: Chr18, 18.89 cM
• Alliance: Ctnna1^em1Cgot page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Using CRISPR/Cas9 endonuclease-mediated genome editing, guide RNAs (G19F 5'-TTAATACGACTCACTATAGGGGCTGCAGACCCCCGAGGAGTGTTTTAGAGCTAGAAATAGCAAGT-3' and G21F 5'-TTAATACGACTCACTATAGGGTCAGCTGGTCATCTTCTGTCGTTTTAGAGCTAGAAATAGCAAGT-3'), were used to target exon 14 of the catenin alpha 1 (Ctnna1) gene to introduce the 4 codon mutations (S641A, S652A, S655A, T658A) in exon 14. The allele harboring 3 of the 4 mutations (CK1 sites, S652A, S655A, T658) was generated. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ctnna1 Mutation: 134 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 1 reference(s)