Mrps5^tm2.2Dshc
Targeted Allele Detail

Summary

• Symbol: Mrps5^tm2.2Dshc
• Name: mitochondrial ribosomal protein S5; targeted mutation 2.2, Dimitri Shcherbakov
• MGI ID: MGI:8268258
• Synonyms: Mrps5^V338Y
• Gene: Mrps5 Location: Chr2:127429346-127445906 bp, + strand Genetic Position: Chr2, 62.05 cM, cytoband F3
• Alliance: Mrps5^tm2.2Dshc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267202
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: This allele was created using the FLEx switch system. A loxP site was inserted upstream of exon 10, a lox511 site, inverted exon 10 containing a valine to tyrosine substitution at amino acid 338 (V338Y), an FRT flanked neomycin selection cassette, loxP site in reverse orientation, and a lox511 site in reverse orientation were inserted downstream of exon 10. The neomycin cassette was removed via flp-mediated recombination. Cre-mediated recombination in the germline removed the wild-type exon 10 and flipped the duplicated inverted exon bearing the V338Y mutation resulting in expression of the mutant allele. Overall expression level of mutant mRNA in the brain, inner ear and retina of homozygous mutant mice is comparable to that in wild-type mice, indicating no deregulation of Mrps5 gene expression. (J:267202)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mrps5 Mutation: 43 strains or lines available

References

• Original: J:267202 Akbergenov R, et al., Mutant MRPS5 affects mitoribosomal accuracy and confers stress-related behavioral alterations. EMBO Rep. 2018 Nov;19(11)
• All: 3 reference(s)