Ehmt2^em1Jchw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ehmt2^em1Jchw
• Name: euchromatic histone lysine N-methyltransferase 2; endonuclease-mediated mutation 1, Jen-Chywan Wang
• MGI ID: MGI:8266917
• Synonyms: Ehmt2^K182R
• Gene: Ehmt2 Location: Chr17:35117445-35133028 bp, + strand Genetic Position: Chr17, 18.45 cM
• Alliance: Ehmt2^em1Jchw page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Lysine codon 182 (AAA) was changed to arginine (AGG) (p.K182R in NP_671493.1 or ENSMUSP00000077208) using an sgRNA (equivalent to AGGTTTGGACATGGTTTTCCNGG) and an ssODN template with CRISPR/Cas9 technology. The mutation reduces the transcriptional coactivation activity (with glucocorticoid receptor (GR)) of the encoded protein, but not its corepression function, which leads to increased dexamethasone-induced insulin resistance. (J:363039)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ehmt2 Mutation: 57 strains or lines available

References

• Original: J:363039 Lee RA, et al., Transcriptional coactivation by EHMT2 restricts glucocorticoid-induced insulin resistance in a study with male mice. Nat Commun. 2023 May 30;14(1):3143
• All: 1 reference(s)