Car4^{em1(icre/ERT2)Eem}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Car4^{em1(icre/ERT2)Eem}
• Name: carbonic anhydrase 4; endonuclease-mediated mutation 1, Edward E Morrisey
• MGI ID: MGI:8255184
• Synonyms: Car4^creERT2
• Gene: Car4 Location: Chr11:84848612-84856870 bp, + strand Genetic Position: Chr11, 51.34 cM
• Alliance: Car4^{em1(icre/ERT2)Eem} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Car4^{em1(icre/ERT2)Eem} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Car4 (MGI:1096574)

• Mutation details: The endogenous stop codon of Car4 was replaced with T2A-iCre/ERT2, followed by a 3xSTOP sequence (tgactgactgat), endogenous polyA signal, 3' regulatory elements, and FRT-flanked Neomycin resistance cassette, directed by a guide RNA (gRNA) targeting the genomic sequence. Double-stranded donor DNA containing the T2A-iCre/ERT2 construct (an optimized variant of Cre recombinase [iCre; improved with mammalian codon usage] fused to Cre-ER(T2) [a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain]) and flanking homologous arms was co-injected with gRNA and Cas9 protein into (C57BL/6 x 129S4/SvJae)F1 derived v6.5 mouse embryonic stem (ES) cells. Flp recombinase removed the frt-flanked neo cassette. (J:101977)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Car4 (mouse) Summary of all recombinase alleles driven by Car4. Alliance mouse Car4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Car4 Mutation: 24 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)