Tbx3^{em1(cre/ERT2)Qfw}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tbx3^{em1(cre/ERT2)Qfw}
• Name: T-box 3; endonuclease-mediated mutation 1, Qing-Feng Wu
• MGI ID: MGI:8254780
• Synonyms: Tbx3-CreER^T2
• Gene: Tbx3 Location: Chr5:119808734-119822789 bp, + strand Genetic Position: Chr5, 60.34 cM
• Alliance: Tbx3^{em1(cre/ERT2)Qfw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:331806
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tbx3^{em1(cre/ERT2)Qfw} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tbx3 (MGI:98495)

• Mutation details: CRISPR/cas9-mediated recombination inserted a cre/ERT2 cassette and and FRT-flanked PGK-puro cassette in place of the coding region of exon 2 (J:331806)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tbx3 (mouse) Summary of all recombinase alleles driven by Tbx3. Alliance mouse Tbx3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbx3 Mutation: 51 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:331806 Shi X, et al., Hierarchical deployment of Tbx3 dictates the identity of hypothalamic KNDy neurons to control puberty onset. Sci Adv. 2022 Nov 18;8(46):eabq2987
• All: 2 reference(s)