Gt(ROSA)26Sor^{em1.1(CAG-RAPGEF3*/mTurquoise2/Venus)Jaffe}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1.1(CAG-RAPGEF3*/mTurquoise2/Venus)Jaffe}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1.1, Laurinda Jaffe
• MGI ID: MGI:8253177
• Synonyms: Gt(ROSA)26Sor^{em1.1(CAG-cAMPFIRE-M)Jaffe}, Rosa26^{CAG-cAMPFIRE-M}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1.1(CAG-RAPGEF3*/mTurquoise2/Venus)Jaffe} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details:

  CRISPR-targeting inserted a CAG-LSL-cAMPFIRE conditional overexpression cassette into the locus. The cAMP Fluorescence Imaging Reporters based on Epac (cAMPFIREs) is composed of a modified RAPGEF3 (M312L, L777A, K778A), mTurquoise2, and Venus (Addgene 182284). Cre-mediated recombination removed the floxed stop cassette to allow for expression of the reporter. (J:376676)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1197 strains or lines available

References

• Original: J:376676 Nakashima IF, et al., Intercellular diffusion of cyclic nucleotides followed by gap junction closure restarts meiosis in mouse preovulatory follicles. Proc Natl Acad Sci U S A. 2025 Dec 9;122(49):e2524136122
• All: 1 reference(s)