Col1a1^{tm10(tetO-Myod1)Hoch}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm10(tetO-Myod1)Hoch}
• Name: collagen, type I, alpha 1; targeted mutation 10, Konrad Hochedlinger
• MGI ID: MGI:8249437
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm10(tetO-Myod1)Hoch} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:372618
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details:

  The targeting vector is designed to insert an FRT-flanked PGK-Neo-pA 500 bp downstream of the 3' UTR of the Col1a1 locus. The FRT-flanked PGK-Neo-pA in the 3' UTR of the Col1a1 locus is then replaced by co-injection of a tetO-MyoD plasmid and Flpe recombinase. The tetO-MyoD plasmid contained the tetracycline responsive element (tetO) upstream of myogenic differentiation 1 (Myod1) cDNA sequence. (J:101977, J:372618)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 170 strains or lines available

References

• Original: J:372618 Yagi M, et al., Dissecting dual roles of MyoD during lineage conversion to mature myocytes and myogenic stem cells. Genes Dev. 2021 Sep 1;35(17-18):1209-1228
• All: 2 reference(s)