Ifnb1^{em1(tdTomato,-cre)Stsn}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ifnb1^{em1(tdTomato,-cre)Stsn}
• Name: interferon beta 1, fibroblast; endonuclease-mediated mutation 1, Daniel Stetson
• MGI ID: MGI:8249192
• Synonyms: Ifnb1-Tomato-Cre-pA Terminator, I-TOMCAT
• Gene: Ifnb1 Location: Chr4:88440262-88441011 bp, - strand Genetic Position: Chr4, 41.91 cM
• Alliance: Ifnb1^{em1(tdTomato,-cre)Stsn} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (No functional change, Recombinase, Reporter)
• Mutation: Insertion
• Ifnb1^{em1(tdTomato,-cre)Stsn} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ifnb1 (MGI:107657)

• Mutation details: A construct containing a encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES), the coding sequence for the TdTomato red fluorescent protein, a picornavirus T2A self-cleaving peptide, a nuclear localization sequence (NLS)-containing Cre recombinase, and a bovine growth hormone (BGH) polyadenylation (pA) sequence was targeted immediately downstream of the endogenous stop codon in ES cells using CRISPR/Cas9 genome editing technology. (J:343796)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ifnb1 (mouse) Summary of all recombinase alleles driven by Ifnb1. Alliance mouse Ifnb1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ifnb1 Mutation: 20 strains or lines available

References

• Original: J:343796 Kotov DI, et al., Early cellular mechanisms of type I interferon-driven susceptibility to tuberculosis. Cell. 2023 Nov 18
• All: 4 reference(s)