Npsr1^{tm1.1(cre/GFP)Ldl}
Targeted Allele Detail

Summary

• Symbol: Npsr1^{tm1.1(cre/GFP)Ldl}
• Name: neuropeptide S receptor 1; targeted mutation 1.1, Luis de Lecea
• MGI ID: MGI:8249167
• Synonyms: Npsr1^Cre
• Gene: Npsr1 Location: Chr9:24009292-24227694 bp, + strand Genetic Position: Chr9, 9.99 cM
• Alliance: Npsr1^{tm1.1(cre/GFP)Ldl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:355278
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion
• Npsr1^{tm1.1(cre/GFP)Ldl} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ntsr1 (MGI:97386)

• Mutation details: A cassette encoding IRES-mnCre:GFP (internal ribosome entry sequence (IRES), a Myc-tag, and nuclear localization signals at the N-terminus of Cre recombinase, which is fused to green fluorescent protein followed by a SV40 polyadenylation sequence) was inserted just 3 of the termination codon in the last coding exon of the gene. A co-inserted frt-flanked neomycin selection was cassette was removed via FLP-mediated recombination in the germline. (J:355278)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ntsr1 (mouse) Summary of all recombinase alleles driven by Ntsr1. Alliance mouse Ntsr1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Npsr1 Mutation: 38 strains or lines available

References

• Original: J:355278 Angelakos CC, et al., A cluster of neuropeptide S neurons regulates breathing and arousal. Curr Biol. 2023 Dec 18;33(24):5439-5455.e7
• All: 1 reference(s)