Clec4f^{em1(cre,-HBEGF)Fhe}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Clec4f^{em1(cre,-HBEGF)Fhe}
• Name: C-type lectin domain family 4, member f; endonuclease-mediated mutation 1, Fuchu He
• MGI ID: MGI:8249135
• Synonyms: Clec4f^Cre/DTR
• Gene: Clec4f Location: Chr6:83621524-83633098 bp, - strand Genetic Position: Chr6, 35.94 cM
• Alliance: Clec4f^{em1(cre,-HBEGF)Fhe} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Clec4f^{em1(cre,-HBEGF)Fhe} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Clec4f (MGI:1859834)

• Mutation details: CRISPR/Cas9-mediated genome editing was used to insert an expression cassette encoding an internal ribosomal entry site (IRES), the Cre enzyme, a self-cleaving 2A peptide, and the human diphtheria toxin receptor (DTR, HBEGF) into the 3-UTR of the Clec4f gene. (J:322626)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Clec4f (mouse) Summary of all recombinase alleles driven by Clec4f. Alliance mouse Clec4f gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clec4f Mutation: 26 strains or lines available

References

• Original: J:322626 Zhao D, et al., ALK1 signaling is required for the homeostasis of Kupffer cells and prevention of bacterial infection. J Clin Invest. 2022 Feb 1;132(3):e150489
• All: 2 reference(s)