Gprc5a^{em1(icre/ERT2)Blr}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gprc5a^{em1(icre/ERT2)Blr}
• Name: G protein-coupled receptor, family C, group 5, member A; endonuclease-mediated mutation 1, Bryan L Roth
• MGI ID: MGI:8246798
• Gene: Gprc5a Location: Chr6:135042660-135061707 bp, + strand Genetic Position: Chr6, 65.83 cM
• Alliance: Gprc5a^{em1(icre/ERT2)Blr} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Gprc5a^{em1(icre/ERT2)Blr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gprc5a (MGI:1891250)

• Mutation details: Using CRISPR/Cas9 endonuclease-mediated genome editing, a guide RNA (5g89T [5'-TGTCCCTTTCGGGGTGATCG-3'] and 3g68T [5'-AAAGGCGACAGCTAACTGTT-3']) were used to introduce an enhanced green fluorescent protein (EGFP) sequence into the C terminal tail and an IRES-iCre/ERT2 (a codon-improved Cre recombinase fused to a human estrogen receptor ligand binding domain) cassette into the 3' UTR of the G protein-coupled receptor, family C, group 5, member A (Gprc5a) gene. (J:94077)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gprc5a (mouse) Summary of all recombinase alleles driven by Gprc5a. Alliance mouse Gprc5a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gprc5a Mutation: 46 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 1 reference(s)