Ntsr1^{tm2.1(cre/GFP)Rpa}
Targeted Allele Detail

Summary

• Symbol: Ntsr1^{tm2.1(cre/GFP)Rpa}
• Name: neurotensin receptor 1; targeted mutation 2.1, Richard D Palmiter
• MGI ID: MGI:8244852
• Synonyms: Ntsr1-frtFLEX-Cre
• Gene: Ntsr1 Location: Chr2:180141769-180186772 bp, + strand Genetic Position: Chr2, 103.04 cM, cytoband H1-H4
• Alliance: Ntsr1^{tm2.1(cre/GFP)Rpa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:349993
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: FLP recombinase
• Mutation: Insertion
• Ntsr1^{tm2.1(cre/GFP)Rpa} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ntsr1 (MGI:97386)

• Mutation details: A FLP-dependent Cre recombinase was constructed by inserting a Mt1 gene intron within the open reading frame of mnCre:GFP, which has a Myc tag (m), followed by a nuclear localization signal (NLS, n), followed by Cre recombinase fused to green fluorescent protein (Cre:GFP), to generate a gene with two exons. Then the second exon was inverted and flanked by a pair of dissimilar frt sites to make a double inverted orientation (DIO) construct (denoted as FLEX). The action of FLP recombinase inverts the second exon thereby allowing splicing to generate functional Cre recombinase. The construct was inserted in-frame to the Ntsr1 gene. A neomycin selection cassette was removed via flp-mediated recombination. (J:349993)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ntsr1 (mouse) Summary of all recombinase alleles driven by Ntsr1. Alliance mouse Ntsr1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ntsr1 Mutation: 32 strains or lines available

References

• Original: J:349993 Heymann G, et al., Synergy of Distinct Dopamine Projection Populations in Behavioral Reinforcement. Neuron. 2020 Mar 4;105(5):909-920.e5
• All: 1 reference(s)