Ldhb^tm1.2Choy
Targeted Allele Detail

Summary

• Symbol: Ldhb^tm1.2Choy
• Name: lactate dehydrogenase B; targeted mutation 1.2, Yun-Hoon Choung
• MGI ID: MGI:8242645
• Gene: Ldhb Location: Chr6:142435975-142453683 bp, - strand Genetic Position: Chr6, 74.17 cM
• Alliance: Ldhb^tm1.2Choy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:371573
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked pgk-neo cassette and loxP site were inserted upstream of exon 3 and a second loxP site was inserted downstream of exon 3 via homologous recombination. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination removed exon 3. Western blot analysis confirmed the absence of expression in heart and kidney from homozygous mice. (J:371573)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ldhb Mutation: 34 strains or lines available

References

• Original: J:371573 Tian C, et al., Suppressed expression of LDHB promotes age-related hearing loss via aerobic glycolysis. Cell Death Dis. 2020 May 15;11(5):375
• All: 3 reference(s)