Dis3l^em1Adki
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dis3l^em1Adki
• Name: DIS3 like exosome 3'-5' exoribonuclease; endonuclease-mediated mutation 1, Andrzej Dziembowski
• MGI ID: MGI:8239625
• Synonyms: DIS3L^GFP
• Gene: Dis3l Location: Chr9:64214038-64248570 bp, - strand Genetic Position: Chr9, 34.62 cM
• Alliance: Dis3l^em1Adki page

Mutation origin

• Strain of Origin: (C57BL/6JTar x CBA/WTar)F1

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: CRISPR-targeting inserted a GFP reporter gene at the 3' end. sgRNA was designed to cut the DNA exactly before the stop codon of exon 17, and the GFP sequence was inserted by providing a GFP-encoding DNA repair template with 60 amino acid-long overhangs facilitating homology-directed repair. (J:365445)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dis3l Mutation: 49 strains or lines available

References

• Original: J:365445 Brouze M, et al., DIS3L, cytoplasmic exosome catalytic subunit, is essential for development but not cell viability in mice. RNA. 2025 Feb 7
• All: 1 reference(s)