Acss2^em1Slbr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Acss2^em1Slbr
• Name: acyl-CoA synthetase short-chain family member 2; endonuclease-mediated mutation 1, Shelley L Berger
• MGI ID: MGI:8237757
• Synonyms: Acss2^KO
• Gene: Acss2 Location: Chr2:155359963-155404663 bp, + strand Genetic Position: Chr2, 77.26 cM, cytoband H2
• Alliance: Acss2^em1Slbr page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR-targeting deleted exons 3 through 7. The 2933 bp deletion introduces multiple premature stop codons, with the first one located at the new junction between exon 2 and exon 8. Western blot analysis confirmed the complete loss of protein across all tissues, including cerebellum and liver. (J:346349)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Acss2 Mutation: 56 strains or lines available

References

• Original: J:346349 Alexander DC, et al., Targeting acetyl-CoA metabolism attenuates the formation of fear memories through reduced activity-dependent histone acetylation. Proc Natl Acad Sci U S A. 2022 Aug 9;119(32):e2114758119
• All: 1 reference(s)