Car1^{em1(cre/ERT2)Cya}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Car1^{em1(cre/ERT2)Cya}
• Name: carbonic anhydrase 1; endonuclease-mediated mutation 1, Cyagen Biosciences
• MGI ID: MGI:8235519
• Synonyms: Car1^{em1(IRES-CreERT2-P2A-EGFP)}
• Gene: Car1 Location: Chr3:14831274-14873425 bp, - strand Genetic Position: Chr3, 3.18 cM
• Alliance: Car1^{em1(cre/ERT2)Cya} page

Mutation origin

• Strain of Origin: C57BL/6JCya

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Car1^{em1(cre/ERT2)Cya} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Car1 (MGI:88268)

• Mutation details: The IRES-CreERT2-P2A-EGFP-WPRE-BGH pA cassette was inserted 60 to 100bp downstream of the TGA stop codon. CreERT2 recombinase and enhanced green fluorescent protein (EGFP) are expressed under the regulatory control of the endogenous gene elements. This model is a Tamoxifen-inducible Cre mouse, and when crossed with allele containing loxP sites, the offspring allele are expected to undergo sequence recombination between loxP sites mediated by Cre recombinase in Car1-positive cells following Tamoxifen induction. In addition to studies of Cre recombinase-mediated gene recombination, this model can also be used for EGFP fluorescent protein tracing studies. (J:326541)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Car1 (mouse) Summary of all recombinase alleles driven by Car1. Alliance mouse Car1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Car1 Mutation: 12 strains or lines available

References

• Original: J:326541 Cyagen Biosciences Inc., Cyagen Biosciences Website. 2022
• All: 1 reference(s)