Ddi2^em1Rase
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ddi2^em1Rase
• Name: DNA-damage inducible protein 2; endonuclease-mediated mutation 1, Radislav Sedlacek
• MGI ID: MGI:8224165
• Synonyms: Ddi2^em1, Ddi2^ex6-
• Gene: Ddi2 Location: Chr4:141410874-141450730 bp, - strand Genetic Position: Chr4, 74.69 cM
• Alliance: Ddi2^em1Rase page

Mutation origin

• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: TALEN-mediated genome editing was used to generate a deletion of exon 6, which results in the ablation of the DDI2 protease domain (delta254-296) of the encoded protein. Immunoblot analysis showed that the DDI2^ex6 protein is scarcely detectable in tissue lysates from E10.5 homozygous embryos. Biophysical characterization of the DDI2^ex6 protein using nuclear magnetic resonance (NMR) showed protein signals of acquired secondary structures; however, the melting temperature of DDI2^ex6 could not be measured by differential scanning fluorimetry (DSF), indicating that, in vivo, the DDI2^ex6 protein likely undergoes rapid degradation. (J:354587)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ddi2 Mutation: 62 strains or lines available

References

• Original: J:354587 Nedomova M, et al., DDI2 protease controls embryonic development and inflammation via TCF11/NRF1. iScience. 2024 Oct 18;27(10):110893
• All: 1 reference(s)