Rho^{tm6.1(RHO*/TagRFP-T)Jhw}
Targeted Allele Detail

Summary

• Symbol: Rho^{tm6.1(RHO*/TagRFP-T)Jhw}
• Name: rhodopsin; targeted mutation 6.1, John H Wilson
• MGI ID: MGI:8221592
• Synonyms: P23H-hRho-TagRFP
• Gene: Rho Location: Chr6:115908709-115916997 bp, + strand Genetic Position: Chr6, 53.72 cM
• Alliance: Rho^{tm6.1(RHO*/TagRFP-T)Jhw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:346249
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The endogenous gene was replaced with a human RHO gene with a P23H mutation, a C terminal fusion to the fluorescent Tag-RFP-T and a lox511-flanked HPRT minigene via homologous recombination. An additional 1D4 signaling sequence was added to the carboxy-terminus in case the C-terminal RFP fusion tag may inhibit the endogenous Rho 1D4 sequence. Cre mediated recombination removed the HPRT minigene. (J:346249)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rho Mutation: 49 strains or lines available

References

• Original: J:346249 Robichaux MA, et al., Subcellular localization of mutant P23H rhodopsin in an RFP fusion knock-in mouse model of retinitis pigmentosa. Dis Model Mech. 2022 May 1;15(5):dmm049336
• All: 1 reference(s)