F8^tm1.1Len
Targeted Allele Detail

Summary

• Symbol: F8^tm1.1Len
• Name: coagulation factor VIII; targeted mutation 1.1, Michael Lenardo
• MGI ID: MGI:8213039
• Synonyms: F8^TKO
• Gene: F8 Location: ChrX:74216321-74426221 bp, - strand Genetic Position: ChrX, 38.17 cM, cytoband A7-B
• Alliance: F8^tm1.1Len page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:266699
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A double targeting strategy was used to generate a floxed allele. First, a loxP flanked PGK-Neo cassette was inserted upstream of the ATG start in exon 1 and then, a second loxP flanked PGK-Hygro cassette was inserted downstream of the TGA stop exon downstream of exon 26. Cre-mediated recombination deleted exons 1-26 and the selection cassettes. qRT-PCR confirmed the absence of mRNA transcripts in the liver. (J:266699)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any F8 Mutation: 24 strains or lines available

References

• Original: J:266699 Chao BN, et al., Characterization of a genetically engineered mouse model of hemophilia A with complete deletion of the F8 gene. J Thromb Haemost. 2016 Feb;14(2):346-55
• All: 2 reference(s)