Apoe^{tm4.1(APOE_i3*R136S)Hol}
Targeted Allele Detail

Summary

• Symbol: Apoe^{tm4.1(APOE_i3*R136S)Hol}
• Name: apolipoprotein E; targeted mutation 4.1, David M Holtzman
• MGI ID: MGI:8211560
• Synonyms: APOE3ch
• Gene: Apoe Location: Chr7:19430034-19433113 bp, - strand Genetic Position: Chr7, 9.94 cM
• Alliance: Apoe^{tm4.1(APOE_i3*R136S)Hol} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:367775
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Apoe^{tm4.1(APOE_i3*R136S)Hol} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOE (348)

  Apoe	(MGI:88057)

  Expresses isoform 3 with p.R136S variant

• Mutation details: The targeting construct replaced the coding sequence with a loxP site, FRT-flanked neomycin resistance cassette, the human coding sequence for isoform 3 of APOE with the Christchurch variant, p.R136S, with signal peptide, an F3 flanked puromycin resistance cassette, loxP site, and human GH polyadenylation sequence. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette. (J:367775)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Apoe Mutation: 163 strains or lines available

References

• Original: J:367775 Chen Y, et al., APOE3ch alters microglial response and suppresses Abeta-induced tau seeding and spread. Cell. 2024 Jan 18;187(2):428-445.e20
• All: 1 reference(s)