Atp1a1^tm1.1Wax
Targeted Allele Detail

Summary

• Symbol: Atp1a1^tm1.1Wax
• Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide; targeted mutation 1.1, Xiaoliang Wang
• MGI ID: MGI:8207780
• Synonyms: mCBM
• Gene: Atp1a1 Location: Chr3:101483535-101512000 bp, - strand Genetic Position: Chr3, 44.3 cM, cytoband F3
• Alliance: Atp1a1^tm1.1Wax page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:362833
• Parent Cell Line: Duffy (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details:

  A floxed neomycin resistance gene cassette was inserted in intron 3 at a site 886 bp upstream of exon 4 and phenylalanine codons 97 and 100 (TTT) in exon 4 were changed to alanine (GCT) (p.F97A, p.F100A) to disrupt the caveolin binding motif (CBM). In addition three silent base pair substitutions were made in exon 4 to introduce restriction sites PyuII and NheI. Cre-mediated recombination removed the neo cassette. (J:362833)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atp1a1 Mutation: 64 strains or lines available

References

• Original: J:362833 Wang X, et al., A caveolin binding motif in Na/K-ATPase is required for stem cell differentiation and organogenesis in mammals and C. elegans. Sci Adv. 2020 May;6(22):eaaw5851
• All: 3 reference(s)