Nwd1^em2.1Sisa
Endonuclease-mediated Allele Detail

Summary

• Symbol: Nwd1^em2.1Sisa
• Name: NACHT and WD repeat domain containing 1; endonuclease-mediated mutation 2.1, Shin-ichi Sakakibara
• MGI ID: MGI:8203529
• Gene: Nwd1 Location: Chr8:73372865-73443508 bp, + strand Genetic Position: Chr8, 35.08 cM
• Alliance: Nwd1^em2.1Sisa page

Mutation origin

• Strain of Origin: B6;D2-Nwd1^em2Sisa/Sisa

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 5 which was flanked by loxP sites was deleted via cre-mediated recombination. qRT-PCR analysis confirmed the absence of mRNA expression in the liver of homozygotes and RT-PCR showed lack of mRNA expression in the region spanning exon 6 to exon 7. Immunostaining confirmed absence of protein in the liver. (J:363603)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nwd1 Mutation: 74 strains or lines available

References

• Original: J:363603 Yamada S, et al., Induction of MASH-like pathogenesis in the Nwd1(-/-) mouse liver. Commun Biol. 2025 Mar 11;8(1):348
• All: 1 reference(s)