Tg(CAG-tdTomato*,-EGFP*,-cre,-EYFP*,-mCherry*,-flpo)iFlpBen
Transgene Detail

Summary

• Symbol: Tg(CAG-tdTomato*,-EGFP*,-cre,-EYFP*,-mCherry*,-flpo)iFlpBen
• Name: transgene insertion iFlp, Rui Benedito
• MGI ID: MGI:8192471
• Synonyms: FlpMosaics, Tg-iFlp^{MTomato-H2B-GFP-Cre/MYFP-H2B-Cherry-FlpO}
• Transgene: Tg(CAG-tdTomato*,-EGFP*,-cre,-EYFP*,-mCherry*,-flpo)iFlpBen Location: unknown Genetic Position: Chr3, Syntenic
• Alliance: Tg(CAG-tdTomato*,-EGFP*,-cre,-EYFP*,-mCherry*,-flpo)iFlpBen page

Transgene origin

• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Transgene description

• Transgene Type: Transgenic (Conditional ready, Recombinase, Reporter)
• Mutation: Insertion
• Tg(CAG-tdTomato*,-EGFP*,-cre,-EYFP*,-mCherry*,-flpo)iFlpBen expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: The transgene contains a CAG promoter, FRT F3 site, FRT ST2 site, neomycin selection cassette, two polyadenylation sequences, FRT F3 site, Mb-Tomato gene (non-oligomerizing tandem-dimer DsRed fluorescent protein variant tdTomato with N-terminal 41 amino acid membrane-targeting tag sequence), 2A sequences, nuclear localized EGFP (H2B-GFP), V5 sequence, 2A site, cre recombinase gene, polyadenylation sequence, FRT ST2 site, MbYFP gene (monomeric enhanced yellow fluorescent protein [mYFP^A206K] with N-terminal membrane-targeting tag sequence), 2A sequences, H2B-Cherry gene (monomeric cherry with 127 amino acid human histone 2B chromatin/nucleus tag and KOZAK sequence plus N-terminal HA tag sequence), 2A sequence, flpo recombinase gene, and polyadenylation sequence. (J:366224)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:366224 Garcia-Gonzalez I, et al., iFlpMosaics enable the multispectral barcoding and high-throughput comparative analysis of mutant and wild-type cells. Nat Methods. 2025 Feb;22(2):323-334
• All: 1 reference(s)